Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 43666..44405 Replicon plasmid p54569CZ_VIM
Accession NZ_CP085763
Organism Enterobacter hormaechei strain 54569CZ

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag LJU41_RS22585 Protein ID WP_013087172.1
Coordinates 43666..44151 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag LJU41_RS22590 Protein ID WP_012540086.1
Coordinates 44139..44405 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU41_RS22565 (LJU41_22565) 39655..39924 + 270 WP_004196355.1 nickel-sensing transcriptional repressor NcrB -
LJU41_RS22570 (LJU41_22570) 39937..41067 + 1131 WP_004196366.1 Ni(II)/Co(II) efflux transporter permease subunit NcrC -
LJU41_RS22575 (LJU41_22575) 41229..41651 + 423 WP_032072095.1 nickel resistance OB fold protein NcrY -
LJU41_RS22580 (LJU41_22580) 41907..43247 - 1341 WP_000589001.1 ISNCY family transposase -
LJU41_RS22585 (LJU41_22585) 43666..44151 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
LJU41_RS22590 (LJU41_22590) 44139..44405 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
LJU41_RS22595 (LJU41_22595) 44706..45029 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
LJU41_RS22600 (LJU41_22600) 45031..45744 + 714 WP_013087170.1 arsenical resistance protein ArsH -
LJU41_RS22605 (LJU41_22605) 45753..46298 + 546 WP_013087169.1 sigma-70 family RNA polymerase sigma factor -
LJU41_RS22610 (LJU41_22610) 46374..46736 + 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
LJU41_RS22615 (LJU41_22615) 46757..47374 + 618 Protein_56 TRC40/GET3/ArsA family transport-energizing ATPase -
LJU41_RS22620 (LJU41_22620) 47355..47912 - 558 WP_243457819.1 ParB N-terminal domain-containing protein -
LJU41_RS22625 (LJU41_22625) 48184..48549 - 366 WP_013087155.1 hypothetical protein -
LJU41_RS22630 (LJU41_22630) 48593..48865 - 273 WP_013087154.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / ant(3'')-Ia / blaVIM-1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / dfrA14 / qnrB56 - 1..171616 171616


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T221240 WP_013087172.1 NZ_CP085763:c44151-43666 [Enterobacter hormaechei]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T221240 NZ_CP117365:312743-313111 [Salmonella enterica subsp. enterica serovar Newport]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTATCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAACAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT221240 WP_012540086.1 NZ_CP085763:c44405-44139 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT221240 NZ_CP117365:312525-312746 [Salmonella enterica subsp. enterica serovar Newport]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTAACGGCGGGCCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGAAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References