Detailed information of TA system TA221224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 525544..526283
• Replicon: chromosome
• Accession: NZ_CP085757
• Organism: Enterobacter hormaechei strain 54569CZ

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3L9PBP9
• Locus tag: LJU41_RS02640
• Protein ID: WP_003857133.1
• Coordinates: 525544..526029 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A927HKN6
• Locus tag: LJU41_RS02645
• Protein ID: WP_045336276.1
• Coordinates: 526017..526283 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU41_RS02610 (LJU41_02610)	521035..521607	+	573	WP_045346197.1	NAD(P)H-dependent oxidoreductase	-
LJU41_RS02615 (LJU41_02615)	521610..522629	+	1020	WP_045346198.1	aldo/keto reductase	-
LJU41_RS02620 (LJU41_02620)	522668..523045	+	378	WP_023292403.1	DUF1330 domain-containing protein	-
LJU41_RS02625 (LJU41_02625)	523139..523612	+	474	Protein_520	NAD(P)H-dependent oxidoreductase	-
LJU41_RS02630 (LJU41_02630)	523728..524423	+	696	WP_045346199.1	antibiotic biosynthesis monooxygenase	-
LJU41_RS02635 (LJU41_02635)	524439..525242	+	804	WP_060579890.1	carboxymuconolactone decarboxylase family protein	-
LJU41_RS02640 (LJU41_02640)	525544..526029	-	486	WP_003857133.1	GNAT family N-acetyltransferase	Toxin
LJU41_RS02645 (LJU41_02645)	526017..526283	-	267	WP_045336276.1	DUF1778 domain-containing protein	Antitoxin
LJU41_RS02650 (LJU41_02650)	526347..527276	-	930	WP_060579891.1	LysR family transcriptional regulator	-
LJU41_RS02655 (LJU41_02655)	527406..528794	+	1389	WP_045336280.1	MFS transporter	-
LJU41_RS02660 (LJU41_02660)	528816..529811	-	996	WP_045336282.1	DUF2891 domain-containing protein	-
LJU41_RS02665 (LJU41_02665)	529821..530807	-	987	WP_003857123.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T221224 WP_003857133.1 NZ_CP085757:c526029-525544 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T221224 NZ_CP117362:824528-824941 [Salmonella enterica subsp. enterica serovar Dublin]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGTCAACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.46 Da
• Isoelectric Point: 4.4857

>AT221224 WP_045336276.1 NZ_CP085757:c526283-526017 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWGV

Nucleotide

• Length: 267 bp

>AT221224 NZ_CP117362:824281-824547 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A3L9PBP9