Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93639..95262 Replicon plasmid p57816CZ_MCR_9
Accession NZ_CP085755
Organism Enterobacter hormaechei strain 57816CZ

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LJU43_RS24805 Protein ID WP_000088044.1
Coordinates 93937..95262 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LJU43_RS24800 Protein ID WP_012477377.1
Coordinates 93639..93932 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU43_RS24780 (LJU43_24780) 89438..89830 + 393 WP_000341066.1 hypothetical protein -
LJU43_RS24785 (LJU43_24785) 90489..91145 + 657 WP_000462754.1 trypsin-like serine protease -
LJU43_RS24790 (LJU43_24790) 91348..91845 + 498 WP_000340139.1 membrane protein -
LJU43_RS24795 (LJU43_24795) 91850..93238 + 1389 WP_012695445.1 hypothetical protein -
LJU43_RS24800 (LJU43_24800) 93639..93932 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LJU43_RS24805 (LJU43_24805) 93937..95262 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LJU43_RS24810 (LJU43_24810) 95323..95529 + 207 WP_000134171.1 hypothetical protein -
LJU43_RS24815 (LJU43_24815) 95630..96040 + 411 WP_000985909.1 hypothetical protein -
LJU43_RS24820 (LJU43_24820) 96053..96577 + 525 WP_001805097.1 hypothetical protein -
LJU43_RS24825 (LJU43_24825) 96759..97763 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
LJU43_RS24830 (LJU43_24830) 97854..98288 - 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / catA2 / aadA2 / qacE / sul1 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / tet(D) / aac(6')-IIc / ere(A) / sul2 / blaSHV-12 - 1..289297 289297
- flank IS/Tn - - 96759..97763 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T221222 WP_000088044.1 NZ_CP085755:93937-95262 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T221222 NZ_CP117362:310103-310471 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGAGCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT221222 WP_012477377.1 NZ_CP085755:93639-93932 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT221222 NZ_CP117362:309885-310106 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References