Detailed information of TA system TA221221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4945324..4945935
• Replicon: chromosome
• Accession: NZ_CP085753
• Organism: Enterobacter hormaechei strain 57816CZ

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: LJU43_RS24215
• Protein ID: WP_012906750.1
• Coordinates: 4945324..4945503 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LJU43_RS24220
• Protein ID: WP_032634114.1
• Coordinates: 4945525..4945935 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU43_RS24190 (4940348)	4940348..4941064	+	717	WP_017384431.1	ABC transporter ATP-binding protein	-
LJU43_RS24195 (4941230)	4941230..4941700	+	471	WP_045142345.1	hypothetical protein	-
LJU43_RS24200 (4941706)	4941706..4942632	-	927	WP_063436689.1	LysR substrate-binding domain-containing protein	-
LJU43_RS24205 (4943237)	4943237..4943770	-	534	WP_032619510.1	glycosyltransferase family A protein	-
LJU43_RS24210 (4943767)	4943767..4945041	-	1275	WP_032619509.1	ATP-binding protein	-
LJU43_RS24215 (4945324)	4945324..4945503	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
LJU43_RS24220 (4945525)	4945525..4945935	+	411	WP_032634114.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LJU43_RS24225 (4945996)	4945996..4946562	+	567	WP_045342272.1	serine acetyltransferase	-
LJU43_RS24230 (4946566)	4946566..4946988	-	423	WP_045342274.1	tail fiber assembly protein	-
LJU43_RS24235 (4946990)	4946990..4949545	-	2556	WP_181494105.1	phage tail protein	-
LJU43_RS24240 (4949548)	4949548..4950099	-	552	WP_045345258.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4940360..4966242	25882

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T221221 WP_012906750.1 NZ_CP085753:4945324-4945503 [Enterobacter hormaechei]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T221221 NZ_CP117362:200046-200531 [Salmonella enterica subsp. enterica serovar Dublin]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14920.82 Da
• Isoelectric Point: 4.3170

>AT221221 WP_032634114.1 NZ_CP085753:4945525-4945935 [Enterobacter hormaechei]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYTGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Nucleotide

• Length: 411 bp

>AT221221 NZ_CP117362:199771-200058 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4