Detailed information of TA system TA022121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2227519..2228048
• Replicon: chromosome
• Accession: NC_009487
• Organism: Staphylococcus aureus subsp. aureus JH9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAURJH9_RS11425
• Protein ID: WP_000621175.1
• Coordinates: 2227519..2227881 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAURJH9_RS11430
• Protein ID: WP_000948331.1
• Coordinates: 2227878..2228048 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAURJH9_RS11400	2224497..2225267	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
SAURJH9_RS11405	2225242..2225721	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAURJH9_RS11410	2225723..2226049	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAURJH9_RS11415	2226168..2227169	-	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
SAURJH9_RS11425	2227519..2227881	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAURJH9_RS11430	2227878..2228048	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAURJH9_RS11435	2228133..2229281	-	1149	WP_001281154.1	alanine racemase	-
SAURJH9_RS11440	2229347..2229706	-	360	WP_000581197.1	holo-ACP synthase	-
SAURJH9_RS11445	2229710..2230201	-	492	WP_001205912.1	PH domain-containing protein	-
SAURJH9_RS11450	2230188..2231771	-	1584	WP_001294626.1	PH domain-containing protein	-
SAURJH9_RS11455	2231764..2232243	-	480	WP_001287087.1	hypothetical protein	-
SAURJH9_RS11460	2232451..2233011	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T22121 WP_000621175.1 NC_009487:c2227881-2227519 [Staphylococcus aureus subsp. aureus JH9]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T22121 NC_009487:c2227881-2227519 [Staphylococcus aureus subsp. aureus JH9]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT22121 WP_000948331.1 NC_009487:c2228048-2227878 [Staphylococcus aureus subsp. aureus JH9]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT22121 NC_009487:c2228048-2227878 [Staphylococcus aureus subsp. aureus JH9]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1