Detailed information of TA system TA221209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2055306..2055922
• Replicon: chromosome
• Accession: NZ_CP085753
• Organism: Enterobacter hormaechei strain 57816CZ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJU43_RS10020
• Protein ID: WP_045142560.1
• Coordinates: 2055551..2055922 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: LJU43_RS10015
• Protein ID: WP_015569912.1
• Coordinates: 2055306..2055548 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU43_RS09985 (2050365)	2050365..2051165	+	801	WP_045142558.1	DeoR/GlpR family DNA-binding transcription regulator	-
LJU43_RS09990 (2051284)	2051284..2051883	+	600	WP_003861947.1	glucose-1-phosphatase	-
LJU43_RS09995 (2051877)	2051877..2052758	+	882	WP_003861949.1	virulence factor BrkB family protein	-
LJU43_RS10000 (2052755)	2052755..2053192	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
LJU43_RS10005 (2053237)	2053237..2054178	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
LJU43_RS10010 (2054187)	2054187..2055107	-	921	WP_045142559.1	alpha/beta hydrolase	-
LJU43_RS10015 (2055306)	2055306..2055548	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
LJU43_RS10020 (2055551)	2055551..2055922	+	372	WP_045142560.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJU43_RS10025 (2055962)	2055962..2056891	-	930	WP_045142561.1	formate dehydrogenase accessory protein FdhE	-
LJU43_RS10030 (2056888)	2056888..2057523	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
LJU43_RS10035 (2057520)	2057520..2058422	-	903	WP_014072386.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13716.82 Da
• Isoelectric Point: 6.6419

>T221209 WP_045142560.1 NZ_CP085753:2055551-2055922 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRRGHEAKTAAVMGAFEIIDVSRDIAERSVSLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T221209 NZ_CP117359:3545662-3545880 [Salmonella enterica subsp. enterica serovar Dublin]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT221209 WP_015569912.1 NZ_CP085753:2055306-2055548 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT221209 NZ_CP117359:3545260-3545634 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3