Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1856501..1857144 Replicon chromosome
Accession NZ_CP085753
Organism Enterobacter hormaechei strain 57816CZ

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LJU43_RS09015 Protein ID WP_001044770.1
Coordinates 1856501..1856917 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LJU43_RS09020 Protein ID WP_001261282.1
Coordinates 1856914..1857144 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU43_RS08985 (1852487) 1852487..1852846 - 360 WP_166479491.1 DUF6314 family protein -
LJU43_RS08990 (1853144) 1853144..1853632 + 489 WP_004099027.1 DUF417 family protein -
LJU43_RS08995 (1854190) 1854190..1854684 + 495 WP_004099028.1 MarR family winged helix-turn-helix transcriptional regulator -
LJU43_RS09000 (1855081) 1855081..1855242 - 162 WP_227789226.1 hypothetical protein -
LJU43_RS09005 (1855440) 1855440..1856144 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
LJU43_RS09010 (1856209) 1856209..1856427 - 219 Protein_1748 AAA family ATPase -
LJU43_RS09015 (1856501) 1856501..1856917 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LJU43_RS09020 (1856914) 1856914..1857144 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LJU43_RS09025 (1857101) 1857101..1857343 + 243 Protein_1751 hypothetical protein -
LJU43_RS09030 (1857645) 1857645..1857935 + 291 WP_013087126.1 hypothetical protein -
LJU43_RS09035 (1858066) 1858066..1858596 + 531 WP_013087127.1 hypothetical protein -
LJU43_RS09040 (1858615) 1858615..1859391 + 777 WP_015572054.1 site-specific integrase -
LJU43_RS09045 (1859593) 1859593..1859850 + 258 Protein_1755 site-specific integrase -
LJU43_RS09050 (1860094) 1860094..1861104 - 1011 WP_015572055.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1798137..1879679 81542
- flank IS/Tn - - 1855440..1856144 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T221207 WP_001044770.1 NZ_CP085753:c1856917-1856501 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T221207 NZ_CP117359:c987630-987103 [Salmonella enterica subsp. enterica serovar Dublin]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT221207 WP_001261282.1 NZ_CP085753:c1857144-1856914 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT221207 NZ_CP117359:c987917-987627 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References