Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 14153..14892 Replicon plasmid p59732CZ_IncFIB
Accession NZ_CP085751
Organism Enterobacter hormaechei strain 59732CZ

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag LJU46_RS24105 Protein ID WP_013087172.1
Coordinates 14153..14638 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag LJU46_RS24110 Protein ID WP_012540086.1
Coordinates 14626..14892 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU46_RS24060 (9414) 9414..10190 - 777 WP_015572054.1 site-specific integrase -
LJU46_RS24065 (10209) 10209..10739 - 531 WP_013087127.1 hypothetical protein -
LJU46_RS24070 (10870) 10870..11160 - 291 WP_013087126.1 hypothetical protein -
LJU46_RS24075 (11462) 11462..11704 - 243 Protein_13 hypothetical protein -
LJU46_RS24080 (11661) 11661..11891 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
LJU46_RS24085 (11888) 11888..12304 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
LJU46_RS24090 (12378) 12378..12596 + 219 Protein_16 AAA family ATPase -
LJU46_RS24095 (12661) 12661..13365 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
LJU46_RS24100 (13409) 13409..13735 - 327 Protein_18 helix-turn-helix domain-containing protein -
LJU46_RS24105 (14153) 14153..14638 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
LJU46_RS24110 (14626) 14626..14892 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
LJU46_RS24115 (15192) 15192..15515 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
LJU46_RS24120 (15517) 15517..16230 + 714 Protein_22 arsenical resistance protein ArsH -
LJU46_RS24125 (16239) 16239..16784 + 546 WP_013087169.1 sigma-70 family RNA polymerase sigma factor -
LJU46_RS24130 (16860) 16860..17222 + 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
LJU46_RS24135 (17243) 17243..19000 + 1758 WP_013087167.1 arsenical pump-driving ATPase -
LJU46_RS24140 (19125) 19125..19661 + 537 WP_013087166.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..35058 35058
- flank IS/Tn - - 12661..13365 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T221202 WP_013087172.1 NZ_CP085751:c14638-14153 [Enterobacter hormaechei]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T221202 NZ_CP117359:200053-200538 [Salmonella enterica subsp. enterica serovar Dublin]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT221202 WP_012540086.1 NZ_CP085751:c14892-14626 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT221202 NZ_CP117359:199778-200065 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References