Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 11661..12304 | Replicon | plasmid p59732CZ_IncFIB |
| Accession | NZ_CP085751 | ||
| Organism | Enterobacter hormaechei strain 59732CZ | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | LJU46_RS24085 | Protein ID | WP_001044770.1 |
| Coordinates | 11888..12304 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | LJU46_RS24080 | Protein ID | WP_001261282.1 |
| Coordinates | 11661..11891 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LJU46_RS24050 (7701) | 7701..8711 | + | 1011 | WP_015572055.1 | RepB family plasmid replication initiator protein | - |
| LJU46_RS24055 (8955) | 8955..9212 | - | 258 | Protein_9 | site-specific integrase | - |
| LJU46_RS24060 (9414) | 9414..10190 | - | 777 | WP_015572054.1 | site-specific integrase | - |
| LJU46_RS24065 (10209) | 10209..10739 | - | 531 | WP_013087127.1 | hypothetical protein | - |
| LJU46_RS24070 (10870) | 10870..11160 | - | 291 | WP_013087126.1 | hypothetical protein | - |
| LJU46_RS24075 (11462) | 11462..11704 | - | 243 | Protein_13 | hypothetical protein | - |
| LJU46_RS24080 (11661) | 11661..11891 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LJU46_RS24085 (11888) | 11888..12304 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LJU46_RS24090 (12378) | 12378..12596 | + | 219 | Protein_16 | AAA family ATPase | - |
| LJU46_RS24095 (12661) | 12661..13365 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| LJU46_RS24100 (13409) | 13409..13735 | - | 327 | Protein_18 | helix-turn-helix domain-containing protein | - |
| LJU46_RS24105 (14153) | 14153..14638 | - | 486 | WP_013087172.1 | GNAT family N-acetyltransferase | - |
| LJU46_RS24110 (14626) | 14626..14892 | - | 267 | WP_012540086.1 | DUF1778 domain-containing protein | - |
| LJU46_RS24115 (15192) | 15192..15515 | + | 324 | WP_013087171.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| LJU46_RS24120 (15517) | 15517..16230 | + | 714 | Protein_22 | arsenical resistance protein ArsH | - |
| LJU46_RS24125 (16239) | 16239..16784 | + | 546 | WP_013087169.1 | sigma-70 family RNA polymerase sigma factor | - |
| LJU46_RS24130 (16860) | 16860..17222 | + | 363 | WP_013087168.1 | arsenic metallochaperone ArsD family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..35058 | 35058 | |
| - | flank | IS/Tn | - | - | 12661..13365 | 704 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T221201 WP_001044770.1 NZ_CP085751:11888-12304 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T221201 NZ_CP117358:c91162-90857 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT221201 WP_001261282.1 NZ_CP085751:11661-11891 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT221201 NZ_CP117358:c91382-91164 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |