Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4263148..4263768 Replicon chromosome
Accession NZ_CP085748
Organism Enterobacter hormaechei strain 59732CZ

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag LJU46_RS20930 Protein ID WP_015571250.1
Coordinates 4263550..4263768 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag LJU46_RS20925 Protein ID WP_006809850.1
Coordinates 4263148..4263522 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU46_RS20915 (LJU46_20915) 4258275..4259468 + 1194 WP_017694395.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LJU46_RS20920 (LJU46_20920) 4259491..4262637 + 3147 WP_015571248.1 multidrug efflux RND transporter permease subunit AcrB -
LJU46_RS20925 (LJU46_20925) 4263148..4263522 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
LJU46_RS20930 (LJU46_20930) 4263550..4263768 + 219 WP_015571250.1 HHA domain-containing protein Toxin
LJU46_RS20935 (LJU46_20935) 4263977..4264528 + 552 WP_015571251.1 maltose O-acetyltransferase -
LJU46_RS20940 (LJU46_20940) 4264645..4265112 + 468 WP_015571252.1 YlaC family protein -
LJU46_RS20945 (LJU46_20945) 4265084..4266544 - 1461 WP_045142063.1 PLP-dependent aminotransferase family protein -
LJU46_RS20950 (LJU46_20950) 4266646..4267356 + 711 WP_181494061.1 GNAT family protein -
LJU46_RS20955 (LJU46_20955) 4267353..4267493 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LJU46_RS20960 (LJU46_20960) 4267496..4267756 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T221200 WP_015571250.1 NZ_CP085748:4263550-4263768 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T221200 NZ_CP117358:24578-24976 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAGTTTATGCTGGATACTAACATCTGCATTTTCACGATAAAAAACAAACCTGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCGGTCACCCTGATGGAGCTGATTTACGGCGCCGAAAAAAGCCAGATGCCCG
AAAGAAATCTCGCTGTGATAGAGGGGTTTGTTTCCCGACTTGATGTTCTGGATTATGACACGCCGGCCGCCACCCATACG
GGGCAGATCAGGGCAGAGCTTGCCCGTCAGGGACGCCCTGTCGGGCCATTCGATCAGATGATTGCCGGTCACGCACGCAG
CCGGGGACTGATTGTTGTCACGAACAACACCCGGGAGTTTGAACGTGTGGGTGGACTGAGAACTGAGGACTGGAGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT221200 WP_006809850.1 NZ_CP085748:4263148-4263522 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT221200 NZ_CP117358:24351-24578 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGAAACCAGCGTATTTCTCAGTAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCAGTGGCTTTGCCGGAAAATGTAAA
ACGTGTGGAAGTTATCGCTGTCGGGCGAACCCGAATTATCACGCCGGCCGGAGAATCGTGGGATGAATGGTTCGACGGGA
ACAACGTCAGCGCCGATTTTATGGATAACCGGGAACAACCCTCTGTGCAGGAACGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References