Detailed information of TA system TA221184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 131741..132337
• Replicon: chromosome
• Accession: NZ_CP085748
• Organism: Enterobacter hormaechei strain 59732CZ

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7G3EX93
• Locus tag: LJU46_RS00655
• Protein ID: WP_015570164.1
• Coordinates: 131741..132043 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A927HL08
• Locus tag: LJU46_RS00660
• Protein ID: WP_023295755.1
• Coordinates: 132050..132337 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU46_RS00635 (LJU46_00635)	127960..129309	+	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-
LJU46_RS00640 (LJU46_00640)	129403..130347	+	945	WP_015570166.1	ketopantoate/pantoate/pantothenate transporter PanS	-
LJU46_RS00645 (LJU46_00645)	130398..130670	+	273	WP_017382612.1	DUF3811 domain-containing protein	-
LJU46_RS00650 (LJU46_00650)	130671..131543	-	873	WP_003860347.1	23S rRNA pseudouridine(2604) synthase RluF	-
LJU46_RS00655 (LJU46_00655)	131741..132043	+	303	WP_015570164.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJU46_RS00660 (LJU46_00660)	132050..132337	+	288	WP_023295755.1	putative addiction module antidote protein	Antitoxin
LJU46_RS00665 (LJU46_00665)	132334..133965	-	1632	WP_003860350.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11425.18 Da
• Isoelectric Point: 10.3736

>T221184 WP_015570164.1 NZ_CP085748:131741-132043 [Enterobacter hormaechei]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRIYFKDQGNSIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T221184 NZ_CP117356:41810-42091 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10101.59 Da
• Isoelectric Point: 5.7807

>AT221184 WP_023295755.1 NZ_CP085748:132050-132337 [Enterobacter hormaechei]
MHKLTPYDPANALLDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT221184 NZ_CP117356:41569-41820 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A7G3EX93