Detailed information of TA system TA221170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 446961..447700
• Replicon: chromosome
• Accession: NZ_CP085743
• Organism: Enterobacter hormaechei strain 60214CZ

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A3L9PBP9
• Locus tag: LJU47_RS02165
• Protein ID: WP_003857133.1
• Coordinates: 447215..447700 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A927HKN6
• Locus tag: LJU47_RS02160
• Protein ID: WP_045336276.1
• Coordinates: 446961..447227 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU47_RS02140 (LJU47_02140)	442437..443423	+	987	WP_003857123.1	DUF979 domain-containing protein	-
LJU47_RS02145 (LJU47_02145)	443433..444428	+	996	WP_045336282.1	DUF2891 domain-containing protein	-
LJU47_RS02150 (LJU47_02150)	444450..445838	-	1389	WP_045336280.1	MFS transporter	-
LJU47_RS02155 (LJU47_02155)	445968..446897	+	930	WP_060579891.1	LysR family transcriptional regulator	-
LJU47_RS02160 (LJU47_02160)	446961..447227	+	267	WP_045336276.1	DUF1778 domain-containing protein	Antitoxin
LJU47_RS02165 (LJU47_02165)	447215..447700	+	486	WP_003857133.1	GNAT family N-acetyltransferase	Toxin
LJU47_RS02170 (LJU47_02170)	448002..448805	-	804	WP_060579890.1	carboxymuconolactone decarboxylase family protein	-
LJU47_RS02175 (LJU47_02175)	448821..449516	-	696	WP_045346199.1	antibiotic biosynthesis monooxygenase	-
LJU47_RS02180 (LJU47_02180)	449632..450105	-	474	Protein_431	NAD(P)H-dependent oxidoreductase	-
LJU47_RS02185 (LJU47_02185)	450199..450576	-	378	WP_023292403.1	DUF1330 domain-containing protein	-
LJU47_RS02190 (LJU47_02190)	450615..451634	-	1020	WP_045346198.1	aldo/keto reductase	-
LJU47_RS02195 (LJU47_02195)	451637..452209	-	573	WP_045346197.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17540.23 Da
• Isoelectric Point: 9.9658

>T221170 WP_003857133.1 NZ_CP085743:447215-447700 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILARLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T221170 NZ_CP117354:835251-835649 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10032.46 Da
• Isoelectric Point: 4.4857

>AT221170 WP_045336276.1 NZ_CP085743:446961-447227 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWGV

Nucleotide

• Length: 267 bp

>AT221170 NZ_CP117354:835024-835251 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: A0A3L9PBP9