Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39013..39538 Replicon plasmid p51135CZ_IncFIB
Accession NZ_CP085742
Organism Klebsiella pneumoniae strain 51135CZ

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag LJU38_RS27395 Protein ID WP_004197633.1
Coordinates 39233..39538 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag LJU38_RS27390 Protein ID WP_006788213.1
Coordinates 39013..39231 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU38_RS27370 (LJU38_27370) 34949..36226 - 1278 WP_048321652.1 HlyD family secretion protein -
LJU38_RS27375 (LJU38_27375) 36888..37766 + 879 WP_009309916.1 restriction endonuclease -
LJU38_RS27380 (LJU38_27380) 37807..38223 - 417 WP_040155119.1 type II toxin-antitoxin system VapC family toxin -
LJU38_RS27385 (LJU38_27385) 38220..38450 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin -
LJU38_RS27390 (LJU38_27390) 39013..39231 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LJU38_RS27395 (LJU38_27395) 39233..39538 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
LJU38_RS27400 (LJU38_27400) 39767..39907 + 141 WP_162898808.1 hypothetical protein -
LJU38_RS27405 (LJU38_27405) 39957..40292 + 336 WP_009309920.1 hypothetical protein -
LJU38_RS27410 (LJU38_27410) 40471..41451 + 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -
LJU38_RS27415 (LJU38_27415) 41601..41744 + 144 Protein_43 IS110 family transposase -
LJU38_RS27420 (LJU38_27420) 41761..42069 + 309 Protein_44 integrase core domain-containing protein -
LJU38_RS27425 (LJU38_27425) 42700..43440 + 741 WP_001515717.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B - 1..107933 107933


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T221162 WP_004197633.1 NZ_CP085742:39233-39538 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T221162 NZ_CP117352:c14266-13916 [Salmonella enterica subsp. enterica serovar Dublin]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT221162 WP_006788213.1 NZ_CP085742:39013-39231 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT221162 NZ_CP117352:c13911-13609 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References