Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 6978..7714 Replicon plasmid p51135CZ_IncFIB
Accession NZ_CP085742
Organism Klebsiella pneumoniae strain 51135CZ

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LJU38_RS27220 Protein ID WP_003026803.1
Coordinates 6978..7460 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LJU38_RS27225 Protein ID WP_003026799.1
Coordinates 7448..7714 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU38_RS27205 (LJU38_27205) 3186..4189 + 1004 Protein_1 IS110-like element IS4321 family transposase -
LJU38_RS27210 (LJU38_27210) 4713..5345 + 633 WP_001567369.1 hypothetical protein -
LJU38_RS27215 (LJU38_27215) 5374..6777 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LJU38_RS27220 (LJU38_27220) 6978..7460 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LJU38_RS27225 (LJU38_27225) 7448..7714 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LJU38_RS27230 (LJU38_27230) 7959..8276 - 318 WP_224440782.1 cell envelope integrity protein TolA -
LJU38_RS27235 (LJU38_27235) 8330..9310 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
LJU38_RS27240 (LJU38_27240) 9457..10086 + 630 Protein_8 IS3-like element ISKpn34 family transposase -
LJU38_RS27245 (LJU38_27245) 10151..10501 + 351 WP_004200891.1 hypothetical protein -
LJU38_RS27250 (LJU38_27250) 11726..12217 - 492 WP_004200903.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B - 1..107933 107933
- inside IScluster/Tn - - 545..10086 9541


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T221160 WP_003026803.1 NZ_CP085742:c7460-6978 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T221160 NZ_CP117351:c4647811-4647500 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT221160 WP_003026799.1 NZ_CP085742:c7714-7448 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT221160 NZ_CP117351:c4647499-4647209 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References