Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5294318..5294943 Replicon chromosome
Accession NZ_CP085740
Organism Klebsiella pneumoniae strain 51135CZ

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LJU38_RS26050 Protein ID WP_002882817.1
Coordinates 5294318..5294701 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LJU38_RS26055 Protein ID WP_004150355.1
Coordinates 5294701..5294943 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU38_RS26035 (LJU38_26035) 5291684..5292586 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LJU38_RS26040 (LJU38_26040) 5292583..5293218 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LJU38_RS26045 (LJU38_26045) 5293215..5294144 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LJU38_RS26050 (LJU38_26050) 5294318..5294701 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LJU38_RS26055 (LJU38_26055) 5294701..5294943 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LJU38_RS26060 (LJU38_26060) 5295148..5296065 + 918 WP_002882812.1 alpha/beta hydrolase -
LJU38_RS26065 (LJU38_26065) 5296079..5297020 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LJU38_RS26070 (LJU38_26070) 5297065..5297502 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LJU38_RS26075 (LJU38_26075) 5297499..5298359 - 861 WP_002882807.1 virulence factor BrkB family protein -
LJU38_RS26080 (LJU38_26080) 5298353..5298952 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T221159 WP_002882817.1 NZ_CP085740:c5294701-5294318 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T221159 NZ_CP117351:c4338674-4338183 [Salmonella enterica subsp. enterica serovar Dublin]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT221159 WP_004150355.1 NZ_CP085740:c5294943-5294701 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT221159 NZ_CP117351:c4338964-4338671 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCCGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCCTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References