Detailed information of TA system TA221143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 244427..245013
• Replicon: chromosome
• Accession: NZ_CP085740
• Organism: Klebsiella pneumoniae strain 51135CZ

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: LJU38_RS01100
• Protein ID: WP_002920800.1
• Coordinates: 244645..245013 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: LJU38_RS01095
• Protein ID: WP_002920802.1
• Coordinates: 244427..244648 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU38_RS01075 (LJU38_01075)	240584..241510	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LJU38_RS01080 (LJU38_01080)	241507..242784	+	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
LJU38_RS01085 (LJU38_01085)	242781..243548	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LJU38_RS01090 (LJU38_01090)	243550..244263	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LJU38_RS01095 (LJU38_01095)	244427..244648	+	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LJU38_RS01100 (LJU38_01100)	244645..245013	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LJU38_RS01105 (LJU38_01105)	245286..246602	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LJU38_RS01110 (LJU38_01110)	246709..247596	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LJU38_RS01115 (LJU38_01115)	247593..248438	+	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LJU38_RS01120 (LJU38_01120)	248440..249510	+	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	239413..250247	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T221143 WP_002920800.1 NZ_CP085740:244645-245013 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T221143 NZ_CP117348:c4588613-4588302 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT221143 WP_002920802.1 NZ_CP085740:244427-244648 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT221143 NZ_CP117348:c4588301-4588011 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4