Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 140580..141490 Replicon plasmid p59062CZ_IncFIB
Accession NZ_CP085732
Organism Klebsiella pneumoniae strain 59062CZ

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag LJU45_RS28385 Protein ID WP_004144067.1
Coordinates 140580..141050 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag LJU45_RS28390 Protein ID WP_023287184.1
Coordinates 141047..141490 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU45_RS28365 (LJU45_28365) 136806..137774 - 969 WP_074423483.1 IS5 family transposase -
LJU45_RS28370 (LJU45_28370) 138079..138339 + 261 WP_017901409.1 hypothetical protein -
LJU45_RS28375 (LJU45_28375) 138528..139496 - 969 WP_072096747.1 IS5 family transposase -
LJU45_RS28380 (LJU45_28380) 139498..140493 - 996 WP_077254794.1 IS5 family transposase -
LJU45_RS28385 (LJU45_28385) 140580..141050 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
LJU45_RS28390 (LJU45_28390) 141047..141490 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
LJU45_RS28395 (LJU45_28395) 141591..142733 - 1143 Protein_155 translesion error-prone DNA polymerase V subunit UmuC -
LJU45_RS28400 (LJU45_28400) 142786..144132 - 1347 WP_225517490.1 ThiF family adenylyltransferase -
LJU45_RS28405 (LJU45_28405) 144328..145296 - 969 WP_074424072.1 IS5 family transposase -
LJU45_RS28410 (LJU45_28410) 145325..146074 - 750 Protein_158 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / tet(D) - 1..227661 227661
- inside IScluster/Tn - - 136806..148218 11412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T221142 WP_004144067.1 NZ_CP085732:c141050-140580 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T221142 NZ_CP117348:c4168609-4168325 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAAGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT221142 WP_023287184.1 NZ_CP085732:c141490-141047 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT221142 NZ_CP117348:c4168841-4168599 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References