Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 71725..72377 Replicon plasmid p59062CZ_IncFIB
Accession NZ_CP085732
Organism Klebsiella pneumoniae strain 59062CZ

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag LJU45_RS28025 Protein ID WP_017901321.1
Coordinates 71952..72377 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag LJU45_RS28020 Protein ID WP_001261275.1
Coordinates 71725..71955 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU45_RS28015 (LJU45_28015) 68896..71472 + 2577 WP_017901322.1 nuclease domain-containing protein -
LJU45_RS28020 (LJU45_28020) 71725..71955 + 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LJU45_RS28025 (LJU45_28025) 71952..72377 + 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
LJU45_RS28030 (LJU45_28030) 72395..73363 + 969 WP_077254762.1 IS5 family transposase -
LJU45_RS28035 (LJU45_28035) 73422..73958 + 537 Protein_82 integrase core domain-containing protein -
LJU45_RS28040 (LJU45_28040) 74011..74184 + 174 Protein_83 nuclease -
LJU45_RS28045 (LJU45_28045) 74371..75927 + 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
LJU45_RS28050 (LJU45_28050) 76019..76246 - 228 Protein_85 IS3 family transposase -
LJU45_RS28055 (LJU45_28055) 76257..76427 + 171 Protein_86 LysR family transcriptional regulator -
LJU45_RS28060 (LJU45_28060) 76552..76668 - 117 Protein_87 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / tet(D) - 1..227661 227661
- flank IS/Tn - - 72395..73363 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T221140 WP_017901321.1 NZ_CP085732:71952-72377 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T221140 NZ_CP117348:3654573-3654836 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGGCGAGCGTTGTTGTGAAGATCTCCGAGCGGGGTTTGATCCTGATTG
CAGAAACGGATGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTA
AATAATGTGGTGAACGGGAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT221140 WP_001261275.1 NZ_CP085732:71725-71955 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT221140 NZ_CP117348:c3654826-3654416 [Salmonella enterica subsp. enterica serovar Heidelberg]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCATCCGTTTCTGCAATCAGGATCAAACCCCGCTCGGAGATCTTCACAACAACGCTCGCCCCGGTCTCAAATCCCGCTT
CTTCCTGGCAGTCGCCGCTGGGGATTTGTGAGTAACAGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGGCG
CCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAG
CGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCCGG
GGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References