Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4016572..4017382 Replicon chromosome
Accession NZ_CP085729
Organism Klebsiella pneumoniae strain 59062CZ

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LJU45_RS20175 Protein ID WP_004178461.1
Coordinates 4016849..4017382 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LJU45_RS20170 Protein ID WP_002887278.1
Coordinates 4016572..4016838 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU45_RS20150 (LJU45_20150) 4012443..4012787 - 345 WP_021462623.1 cation efflux system protein CusF -
LJU45_RS20155 (LJU45_20155) 4012805..4014190 - 1386 WP_029602902.1 efflux transporter outer membrane subunit -
LJU45_RS20160 (LJU45_20160) 4014363..4015046 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
LJU45_RS20165 (LJU45_20165) 4015036..4016469 + 1434 WP_029602901.1 Cu(+)/Ag(+) sensor histidine kinase -
LJU45_RS20170 (LJU45_20170) 4016572..4016838 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LJU45_RS20175 (LJU45_20175) 4016849..4017382 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LJU45_RS20180 (LJU45_20180) 4017430..4018551 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T221137 WP_004178461.1 NZ_CP085729:4016849-4017382 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T221137 NZ_CP117348:2059542-2059645 [Salmonella enterica subsp. enterica serovar Heidelberg]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT221137 WP_002887278.1 NZ_CP085729:4016572-4016838 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT221137 NZ_CP117348:c2059647-2059504 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References