Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3443017..3443642 Replicon chromosome
Accession NZ_CP085729
Organism Klebsiella pneumoniae strain 59062CZ

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LJU45_RS17410 Protein ID WP_002882817.1
Coordinates 3443259..3443642 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LJU45_RS17405 Protein ID WP_004150355.1
Coordinates 3443017..3443259 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU45_RS17380 (LJU45_17380) 3439008..3439607 + 600 WP_004151865.1 glucose-1-phosphatase -
LJU45_RS17385 (LJU45_17385) 3439601..3440461 + 861 WP_004146232.1 virulence factor BrkB family protein -
LJU45_RS17390 (LJU45_17390) 3440458..3440895 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LJU45_RS17395 (LJU45_17395) 3440940..3441881 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LJU45_RS17400 (LJU45_17400) 3441895..3442812 - 918 WP_004178029.1 alpha/beta hydrolase -
LJU45_RS17405 (LJU45_17405) 3443017..3443259 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LJU45_RS17410 (LJU45_17410) 3443259..3443642 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LJU45_RS17415 (LJU45_17415) 3443816..3444745 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LJU45_RS17420 (LJU45_17420) 3444742..3445377 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LJU45_RS17425 (LJU45_17425) 3445374..3446276 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T221134 WP_002882817.1 NZ_CP085729:3443259-3443642 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T221134 NZ_CP117348:383599-383874 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGGGCCTGACTGGATTCTTGTTGATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT221134 WP_004150355.1 NZ_CP085729:3443017-3443259 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT221134 NZ_CP117348:383336-383596 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGAATCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGGATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGACATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References