Detailed information of TA system TA221119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 497482..498118
• Replicon: chromosome
• Accession: NZ_CP085712
• Organism: Cytobacillus solani strain F27

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W7KPR3
• Locus tag: LIS82_RS02770
• Protein ID: WP_009336311.1
• Coordinates: 497768..498118 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LIS82_RS02765
• Protein ID: WP_053477859.1
• Coordinates: 497482..497763 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS82_RS02745 (LIS82_02750)	493436..494164	-	729	WP_053477863.1	rhomboid family intramembrane serine protease	-
LIS82_RS02750 (LIS82_02755)	494423..494791	+	369	WP_053477862.1	holo-ACP synthase	-
LIS82_RS02755 (LIS82_02760)	495001..496008	+	1008	WP_053477861.1	outer membrane lipoprotein carrier protein LolA	-
LIS82_RS02760 (LIS82_02765)	496146..497303	+	1158	WP_053477860.1	alanine racemase	-
LIS82_RS02765 (LIS82_02770)	497482..497763	+	282	WP_053477859.1	CopG family ribbon-helix-helix protein	Antitoxin
LIS82_RS02770 (LIS82_02775)	497768..498118	+	351	WP_009336311.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LIS82_RS02775 (LIS82_02780)	498369..498701	+	333	WP_056682040.1	anti-sigma factor antagonist	-
LIS82_RS02780 (LIS82_02785)	498698..499171	+	474	WP_053477857.1	anti-sigma B factor RsbW	-
LIS82_RS02785 (LIS82_02790)	499149..499934	+	786	WP_142108196.1	RNA polymerase sigma factor SigB	-
LIS82_RS02790 (LIS82_02795)	500073..502250	+	2178	WP_053477855.1	Tex family protein	-
LIS82_RS02795 (LIS82_02800)	502640..502753	-	114	WP_142108194.1	cortex morphogenetic protein CmpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13006.03 Da
• Isoelectric Point: 4.8998

>T221119 WP_009336311.1 NZ_CP085712:497768-498118 [Cytobacillus solani]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEAVQISLGLIEF

Nucleotide

• Length: 351 bp

>T221119 NZ_CP117346:c987632-987105 [Salmonella enterica subsp. enterica serovar Dublin]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10769.30 Da
• Isoelectric Point: 7.2038

>AT221119 WP_053477859.1 NZ_CP085712:497482-497763 [Cytobacillus solani]
VAESSATTEIMVKLPQHLLTELDGFAKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEF
EAEHTVERLVSGG

Nucleotide

• Length: 282 bp

>AT221119 NZ_CP117346:c987919-987629 [Salmonella enterica subsp. enterica serovar Dublin]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A169FD46