221118

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 245727..246363 Replicon chromosome
Accession NZ_CP085708
Organism Bacillus sp. CMF21

Toxin (Protein)


Gene name mazF Uniprot ID A0A2N5FBL1
Locus tag LIT32_RS01370 Protein ID WP_029286376.1
Coordinates 246013..246363 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A2N5FBR6
Locus tag LIT32_RS01365 Protein ID WP_029286378.1
Coordinates 245727..246008 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIT32_RS01345 (LIT32_01350) 241757..242350 - 594 WP_223439017.1 rhomboid family intramembrane serine protease -
LIT32_RS01350 (LIT32_01355) 242453..242818 + 366 WP_223439018.1 holo-ACP synthase -
LIT32_RS01355 (LIT32_01360) 242964..243974 + 1011 WP_223439019.1 outer membrane lipoprotein carrier protein LolA -
LIT32_RS01360 (LIT32_01365) 244399..245580 + 1182 WP_223439020.1 alanine racemase -
LIT32_RS01365 (LIT32_01370) 245727..246008 + 282 WP_029286378.1 YlcI/YnfO family protein Antitoxin
LIT32_RS01370 (LIT32_01375) 246013..246363 + 351 WP_029286376.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LIT32_RS01375 (LIT32_01380) 246501..247337 + 837 WP_223439021.1 STAS domain-containing protein -
LIT32_RS01380 (LIT32_01385) 247334..247699 + 366 WP_101569209.1 STAS domain-containing protein -
LIT32_RS01385 (LIT32_01390) 247702..248103 + 402 WP_070878720.1 anti-sigma regulatory factor -
LIT32_RS01390 (LIT32_01395) 248115..249125 + 1011 WP_223439022.1 PP2C family protein-serine/threonine phosphatase -
LIT32_RS01395 (LIT32_01400) 249188..249520 + 333 WP_223439023.1 anti-sigma factor antagonist -
LIT32_RS01400 (LIT32_01405) 249517..249999 + 483 WP_223439024.1 anti-sigma B factor RsbW -
LIT32_RS01405 (LIT32_01410) 249965..250753 + 789 WP_070878724.1 RNA polymerase sigma factor SigB -
LIT32_RS01410 (LIT32_01415) 250753..251352 + 600 WP_223439025.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13036.06 Da        Isoelectric Point: 4.8781

>T221118 WP_029286376.1 NZ_CP085708:246013-246363 [Bacillus sp. CMF21]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDSKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T221118 NZ_CP117346:835442-835840 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10572.01 Da        Isoelectric Point: 7.0156

>AT221118 WP_029286378.1 NZ_CP085708:245727-246008 [Bacillus sp. CMF21]
VSESSATTEILIRLPQALVSELDGLVKQENGNRSELIYQATKMYIRERKKRQIRESMRRGYMEMAKINLNIASEAFQAES
EADHTVERLVSGG

Download         Length: 282 bp

>AT221118 NZ_CP117346:835215-835442 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N5FBL1

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5FBR6

References