Detailed information of TA system TA221116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 479006..479643
• Replicon: chromosome
• Accession: NZ_CP085706
• Organism: Bacillus velezensis strain CK17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: LIT36_RS02485
• Protein ID: WP_003156187.1
• Coordinates: 479293..479643 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: LIT36_RS02480
• Protein ID: WP_003156188.1
• Coordinates: 479006..479287 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIT36_RS02460 (LIT36_02470)	475371..475970	-	600	WP_015388676.1	rhomboid family intramembrane serine protease	-
LIT36_RS02465 (LIT36_02475)	476063..476428	+	366	WP_003156192.1	holo-ACP synthase	-
LIT36_RS02470 (LIT36_02480)	476593..477600	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
LIT36_RS02475 (LIT36_02485)	477717..478886	+	1170	WP_003156189.1	alanine racemase	-
LIT36_RS02480 (LIT36_02490)	479006..479287	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
LIT36_RS02485 (LIT36_02495)	479293..479643	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
LIT36_RS02490 (LIT36_02500)	479761..480582	+	822	WP_014304404.1	STAS domain-containing protein	-
LIT36_RS02495 (LIT36_02505)	480587..480952	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
LIT36_RS02500 (LIT36_02510)	480955..481356	+	402	WP_003156178.1	anti-sigma regulatory factor	-
LIT36_RS02505 (LIT36_02515)	481368..482375	+	1008	WP_025853725.1	PP2C family protein-serine/threonine phosphatase	-
LIT36_RS02510 (LIT36_02520)	482439..482768	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
LIT36_RS02515 (LIT36_02525)	482765..483247	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
LIT36_RS02520 (LIT36_02530)	483213..484001	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
LIT36_RS02525 (LIT36_02535)	484001..484603	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T221116 WP_003156187.1 NZ_CP085706:479293-479643 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T221116 NZ_CP117346:358266-358541 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT221116 WP_003156188.1 NZ_CP085706:479006-479287 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT221116 NZ_CP117346:358003-358263 [Salmonella enterica subsp. enterica serovar Dublin]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5