Detailed information of TA system TA221105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4140897..4141473
• Replicon: chromosome
• Accession: NZ_CP085699
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain S29

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: LJN59_RS20340
• Protein ID: WP_001131963.1
• Coordinates: 4141186..4141473 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: LJN59_RS20335
• Protein ID: WP_000063142.1
• Coordinates: 4140897..4141199 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN59_RS20320 (4137407)	4137407..4139557	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
LJN59_RS20325 (4139652)	4139652..4139855	+	204	WP_000460616.1	YbdD/YjiX family protein	-
LJN59_RS20330 (4139866)	4139866..4140822	+	957	WP_000187839.1	GTPase	-
LJN59_RS20335 (4140897)	4140897..4141199	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
LJN59_RS20340 (4141186)	4141186..4141473	-	288	WP_001131963.1	BrnT family toxin	Toxin
LJN59_RS20345 (4141742)	4141742..4142656	-	915	WP_000290512.1	restriction endonuclease	-
LJN59_RS20350 (4142854)	4142854..4146363	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T221105 WP_001131963.1 NZ_CP085699:c4141473-4141186 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T221105 NZ_CP117343:c4195767-4195459 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT221105 WP_000063142.1 NZ_CP085699:c4141199-4140897 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT221105 NZ_CP117343:c4196009-4195770 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01