Detailed information of TA system TA022107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 8119994..8120667
• Replicon: chromosome
• Accession: NC_009485
• Organism: Bradyrhizobium sp. BTAi1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A5ETR9
• Locus tag: BBTA_RS36135
• Protein ID: WP_012047448.1
• Coordinates: 8120245..8120667 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A5ETR8
• Locus tag: BBTA_RS36130
• Protein ID: WP_012047447.1
• Coordinates: 8119994..8120248 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBTA_RS36120	8117595..8118656	+	1062	WP_012047445.1	toprim domain-containing protein	-
BBTA_RS36125	8118991..8119914	+	924	WP_012047446.1	DUF2493 domain-containing protein	-
BBTA_RS36130	8119994..8120248	+	255	WP_012047447.1	plasmid stabilization protein	Antitoxin
BBTA_RS36135	8120245..8120667	+	423	WP_012047448.1	type II toxin-antitoxin system VapC family toxin	Toxin
BBTA_RS36140	8120838..8121278	+	441	WP_041751132.1	hypothetical protein	-
BBTA_RS36145	8121868..8122191	+	324	WP_041751133.1	DUF736 domain-containing protein	-
BBTA_RS36150	8122395..8122646	+	252	WP_012047452.1	helix-turn-helix domain-containing protein	-
BBTA_RS36155	8122767..8123024	+	258	WP_012047453.1	DUF2285 domain-containing protein	-
BBTA_RS41175	8123150..8123470	+	321	WP_012047454.1	hypothetical protein	-
BBTA_RS36160	8123418..8123921	+	504	WP_201160176.1	DUF2285 domain-containing protein	-
BBTA_RS36165	8124059..8124334	+	276	WP_012047456.1	helix-turn-helix domain-containing protein	-
BBTA_RS36170	8124345..8125223	+	879	WP_012047457.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	8092241..8136994	44753

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14862.18 Da
• Isoelectric Point: 9.6550

>T22107 WP_012047448.1 NC_009485:8120245-8120667 [Bradyrhizobium sp. BTAi1]
MILLDTNVVSEAMKPEPHPSVRDWLDAQAAETLFLSSVTIAELMFGIGALPRGKRKDRLATTLDGVMELFKPRVLPFDTQ
AARRYADLAVKARKAGKGFPTPDGYIAAIAAAHGFAVASRHTAAFAAAGLTVIDPWTGGG

Nucleotide

• Length: 423 bp

>T22107 NC_009485:8120245-8120667 [Bradyrhizobium sp. BTAi1]
ATGATCCTGCTCGACACCAATGTCGTCTCCGAGGCGATGAAGCCCGAGCCACATCCGTCTGTCCGGGACTGGCTCGACGC
CCAGGCCGCGGAAACGCTGTTTCTCTCCAGCGTCACCATCGCGGAGCTGATGTTCGGCATCGGCGCGCTGCCCAGGGGCA
AGCGCAAGGACCGCCTGGCAACCACGCTCGACGGCGTGATGGAGCTGTTCAAGCCGAGAGTCCTGCCCTTCGATACGCAA
GCGGCGCGGCGATACGCCGACCTCGCCGTCAAGGCGCGCAAGGCCGGCAAGGGCTTCCCCACGCCCGACGGCTACATCGC
CGCGATCGCCGCCGCGCACGGCTTCGCCGTGGCGTCGCGCCACACCGCCGCGTTCGCGGCCGCCGGCCTGACGGTCATCG
ATCCCTGGACGGGCGGCGGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9261.64 Da
• Isoelectric Point: 11.3253

>AT22107 WP_012047447.1 NC_009485:8119994-8120248 [Bradyrhizobium sp. BTAi1]
MPAVTIRNLSEEAHRALKVRAAQHNRSTEAEMRAILEAAVRPAGRLKIGTALSEMSRKIGLNNADVEAVEQVRDTRPAQP
MRFG

Nucleotide

• Length: 255 bp

>AT22107 NC_009485:8119994-8120248 [Bradyrhizobium sp. BTAi1]
ATGCCCGCTGTCACTATTCGCAACCTCTCGGAGGAGGCGCATCGCGCCCTGAAGGTCCGGGCGGCGCAGCACAACCGCAG
CACGGAAGCCGAAATGCGCGCCATTCTGGAAGCCGCCGTGCGCCCCGCGGGGCGGCTCAAGATCGGCACCGCGCTCTCGG
AGATGAGCCGCAAGATCGGACTCAACAATGCCGATGTCGAGGCGGTCGAACAGGTTCGCGACACCCGGCCCGCCCAGCCG
ATGCGCTTCGGATGA

Structures

Toxin

• AlphaFold DB: A5ETR9

Antitoxin

• AlphaFold DB: A5ETR8