Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4294882..4295513 Replicon chromosome
Accession NZ_CP085638
Organism Escherichia coli strain fEC.1

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag LJX82_RS20805 Protein ID WP_001259388.1
Coordinates 4295238..4295513 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LJX82_RS20800 Protein ID WP_000593555.1
Coordinates 4294882..4295241 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJX82_RS20775 (4291014) 4291014..4291958 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
LJX82_RS20780 (4291955) 4291955..4292788 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LJX82_RS20785 (4292788) 4292788..4293552 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LJX82_RS20790 (4293549) 4293549..4294355 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LJX82_RS20795 (4294361) 4294361..4294762 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LJX82_RS20800 (4294882) 4294882..4295241 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LJX82_RS20805 (4295238) 4295238..4295513 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
LJX82_RS20810 (4295586) 4295586..4296710 - 1125 WP_001314210.1 ABC transporter permease -
LJX82_RS20815 (4296710) 4296710..4299445 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
LJX82_RS20820 (4299442) 4299442..4300509 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T221045 WP_001259388.1 NZ_CP085638:c4295513-4295238 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T221045 NZ_CP117336:835437-835835 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT221045 WP_000593555.1 NZ_CP085638:c4295241-4294882 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT221045 NZ_CP117336:835210-835437 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References