Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3579958..3580541 Replicon chromosome
Accession NZ_CP085638
Organism Escherichia coli strain fEC.1

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag LJX82_RS17255 Protein ID WP_000254745.1
Coordinates 3579958..3580293 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag LJX82_RS17260 Protein ID WP_000581937.1
Coordinates 3580293..3580541 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJX82_RS17240 (3575845) 3575845..3577143 - 1299 WP_000036723.1 phosphopyruvate hydratase -
LJX82_RS17245 (3577231) 3577231..3578868 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
LJX82_RS17250 (3579096) 3579096..3579887 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
LJX82_RS17255 (3579958) 3579958..3580293 - 336 WP_000254745.1 endoribonuclease MazF Toxin
LJX82_RS17260 (3580293) 3580293..3580541 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
LJX82_RS17265 (3580619) 3580619..3582853 - 2235 WP_000226815.1 GTP pyrophosphokinase -
LJX82_RS17270 (3582901) 3582901..3584202 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T221042 WP_000254745.1 NZ_CP085638:c3580293-3579958 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T221042 NZ_CP117336:310106-310474 [Salmonella enterica subsp. enterica serovar Dublin]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGAGCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCACTATATCCCGTGGGTATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT221042 WP_000581937.1 NZ_CP085638:c3580541-3580293 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT221042 NZ_CP117336:309888-310109 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References