Detailed information of TA system TA221041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3283987..3284612
• Replicon: chromosome
• Accession: NZ_CP085638
• Organism: Escherichia coli strain fEC.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJX82_RS15830
• Protein ID: WP_000911330.1
• Coordinates: 3283987..3284385 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LJX82_RS15835
• Protein ID: WP_000450524.1
• Coordinates: 3284385..3284612 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJX82_RS15810 (3279865)	3279865..3280065	+	201	WP_000383836.1	YpfN family protein	-
LJX82_RS15815 (3280175)	3280175..3280873	-	699	WP_000679823.1	esterase	-
LJX82_RS15820 (3280947)	3280947..3282962	-	2016	WP_063112794.1	tRNA cytosine(34) acetyltransferase TmcA	-
LJX82_RS15825 (3282977)	3282977..3283840	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
LJX82_RS15830 (3283987)	3283987..3284385	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJX82_RS15835 (3284385)	3284385..3284612	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LJX82_RS15840 (3284766)	3284766..3285479	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LJX82_RS15845 (3285692)	3285692..3286726	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
LJX82_RS15850 (3286743)	3286743..3287621	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LJX82_RS15855 (3287767)	3287767..3288339	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LJX82_RS15860 (3288339)	3288339..3288809	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T221041 WP_000911330.1 NZ_CP085638:c3284385-3283987 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T221041 NZ_CP117336:200050-200535 [Salmonella enterica subsp. enterica serovar Dublin]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT221041 WP_000450524.1 NZ_CP085638:c3284612-3284385 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT221041 NZ_CP117336:199775-200062 [Salmonella enterica subsp. enterica serovar Dublin]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33