Detailed information of TA system TA022103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 322282..323068
• Replicon: chromosome
• Accession: NC_009484
• Organism: Acidiphilium cryptum JF-5

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A5FV77
• Locus tag: ACRY_RS03870
• Protein ID: WP_011941412.1
• Coordinates: 322577..323068 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: ACRY_RS03865
• Protein ID: WP_148360949.1
• Coordinates: 322282..322575 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACRY_RS03845	317827..318465	+	639	WP_011941407.1	hypothetical protein	-
ACRY_RS03850	318727..319059	+	333	WP_011941408.1	helix-turn-helix domain-containing protein	-
ACRY_RS03855	319056..321113	+	2058	WP_011941409.1	tyrosine-type recombinase/integrase	-
ACRY_RS03860	321118..322200	+	1083	WP_013639294.1	site-specific integrase	-
ACRY_RS03865	322282..322575	+	294	WP_148360949.1	DUF1778 domain-containing protein	Antitoxin
ACRY_RS03870	322577..323068	+	492	WP_011941412.1	GNAT family N-acetyltransferase	Toxin
ACRY_RS03875	323464..324210	-	747	WP_011930761.1	IS21-like element helper ATPase IstB	-
ACRY_RS03880	324228..325754	-	1527	WP_081432832.1	IS21 family transposase	-
ACRY_RS03885	326028..326366	+	339	Protein_284	hypothetical protein	-
ACRY_RS03890	326433..326975	+	543	WP_011941414.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	318799..337404	18605
	-	flank	IS/Tn	-	-	323464..324210	746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (17-91)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17265.87 Da
• Isoelectric Point: 10.1522

>T22103 WP_011941412.1 NC_009484:322577-323068 [Acidiphilium cryptum JF-5]
VALTPPEPLAEHHDISGFNCGIASLDDWLRRRARANQAGGATRTFVVTESGRVVAYYAIASGAISPNGTSGRFRRNMPDP
IPVAVLARLAIATSAQGQGLGRALFRDATLRILNAAETLGIRGIVVHAISDQAKAFYLALGFEASPLDPMTLMVTLKDVR
AAL

Nucleotide

• Length: 492 bp

>T22103 NC_009484:322577-323068 [Acidiphilium cryptum JF-5]
GTGGCCCTCACCCCGCCCGAACCGCTCGCCGAGCATCACGACATCAGCGGCTTCAACTGCGGCATCGCCTCACTGGATGA
CTGGCTGAGGCGCCGGGCCCGCGCCAACCAGGCCGGCGGCGCGACCCGTACCTTCGTTGTGACCGAAAGCGGCCGCGTCG
TCGCCTATTACGCCATCGCTTCCGGCGCCATCAGCCCGAACGGCACCTCCGGCCGCTTCCGCCGCAACATGCCCGACCCG
ATCCCCGTCGCCGTCCTCGCCCGCCTCGCAATCGCCACCAGCGCCCAGGGACAAGGCCTCGGCCGCGCCCTCTTCCGCGA
CGCCACCCTCCGCATCCTGAACGCAGCCGAAACGCTGGGGATCAGAGGCATCGTGGTGCATGCCATCTCCGACCAGGCCA
AAGCCTTCTACCTCGCCCTCGGCTTCGAAGCCTCGCCGCTCGATCCGATGACGCTGATGGTCACGCTCAAGGATGTGAGA
GCGGCATTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10744.20 Da
• Isoelectric Point: 10.5728

>AT22103 WP_148360949.1 NC_009484:322282-322575 [Acidiphilium cryptum JF-5]
MNPAKQASAPSSRRDTLNLRIKSDDRGLIDRAAALTGKTKTDFVIDAARRAAEDALLDRTLFLATPDAFDAFRARLDEPP
LPNARLQRALKTTPPWE

Nucleotide

• Length: 294 bp

>AT22103 NC_009484:322282-322575 [Acidiphilium cryptum JF-5]
ATGAATCCCGCCAAGCAAGCCTCTGCGCCATCCTCCCGCCGCGACACGCTCAACCTGCGGATCAAAAGCGACGATCGCGG
GCTGATCGACCGCGCCGCCGCCCTGACCGGCAAGACCAAGACGGACTTCGTGATCGACGCCGCCCGCCGCGCCGCCGAAG
ACGCCCTGCTCGACCGCACGCTGTTTCTCGCAACTCCGGATGCGTTCGACGCCTTCCGCGCGCGCCTCGACGAACCGCCG
CTGCCGAATGCCCGCCTCCAGCGCGCCCTGAAGACAACGCCGCCCTGGGAATGA

Structures

Toxin

• AlphaFold DB: A5FV77