Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3508602..3509439 Replicon chromosome
Accession NZ_CP085637
Organism Escherichia coli strain fECg99.1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag LJU33_RS16995 Protein ID WP_000227784.1
Coordinates 3508897..3509439 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A0A3T7ZCX8
Locus tag LJU33_RS16990 Protein ID WP_001442339.1
Coordinates 3508602..3508913 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU33_RS16965 (3503622) 3503622..3504569 + 948 WP_001239444.1 cytochrome o ubiquinol oxidase subunit II -
LJU33_RS16970 (3504591) 3504591..3506582 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
LJU33_RS16975 (3506572) 3506572..3507186 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LJU33_RS16980 (3507186) 3507186..3507515 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LJU33_RS16985 (3507527) 3507527..3508417 + 891 WP_000971336.1 heme o synthase -
LJU33_RS16990 (3508602) 3508602..3508913 + 312 WP_001442339.1 DUF1778 domain-containing protein Antitoxin
LJU33_RS16995 (3508897) 3508897..3509439 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
LJU33_RS17000 (3509495) 3509495..3510430 - 936 WP_001297127.1 tetratricopeptide repeat protein -
LJU33_RS17005 (3510838) 3510838..3512202 + 1365 WP_001000983.1 MFS transporter -
LJU33_RS17010 (3512330) 3512330..3512821 - 492 WP_001138904.1 nucleotide binding protein YajQ -
LJU33_RS17015 (3512989) 3512989..3513900 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T221017 WP_000227784.1 NZ_CP085637:3508897-3509439 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T221017 NZ_CP117329:2416210-2416494 [Salmonella enterica subsp. enterica serovar Muenster]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACAACACAGAAGCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT221017 WP_001442339.1 NZ_CP085637:3508602-3508913 [Escherichia coli]
MPGKTATLPDVDKTLKNARVEIKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT221017 NZ_CP117329:2415972-2416220 [Salmonella enterica subsp. enterica serovar Muenster]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A3T7ZCX8

References