Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3474525..3475143 Replicon chromosome
Accession NZ_CP085637
Organism Escherichia coli strain fECg99.1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LJU33_RS16825 Protein ID WP_001291435.1
Coordinates 3474925..3475143 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LJU33_RS16820 Protein ID WP_000344800.1
Coordinates 3474525..3474899 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU33_RS16810 (3469614) 3469614..3470807 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LJU33_RS16815 (3470830) 3470830..3473979 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LJU33_RS16820 (3474525) 3474525..3474899 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LJU33_RS16825 (3474925) 3474925..3475143 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LJU33_RS16830 (3475315) 3475315..3475866 + 552 WP_000102564.1 maltose O-acetyltransferase -
LJU33_RS16835 (3475982) 3475982..3476452 + 471 WP_000136192.1 YlaC family protein -
LJU33_RS16840 (3476616) 3476616..3478166 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LJU33_RS16845 (3478208) 3478208..3478561 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LJU33_RS16855 (3478940) 3478940..3479251 + 312 WP_000409911.1 MGMT family protein -
LJU33_RS16860 (3479282) 3479282..3479854 - 573 WP_023156216.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T221016 WP_001291435.1 NZ_CP085637:3474925-3475143 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T221016 NZ_CP117329:2059467-2059570 [Salmonella enterica subsp. enterica serovar Muenster]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT221016 WP_000344800.1 NZ_CP085637:3474525-3474899 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT221016 NZ_CP117329:c2059572-2059429 [Salmonella enterica subsp. enterica serovar Muenster]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References