Detailed information of TA system TA221005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1315887..1316512
• Replicon: chromosome
• Accession: NZ_CP085637
• Organism: Escherichia coli strain fECg99.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8S7SZU1
• Locus tag: LJU33_RS06360
• Protein ID: WP_000911327.1
• Coordinates: 1316114..1316512 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: LJU33_RS06355
• Protein ID: WP_000450524.1
• Coordinates: 1315887..1316114 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJU33_RS06330 (1311689)	1311689..1312159	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
LJU33_RS06335 (1312159)	1312159..1312731	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
LJU33_RS06340 (1312877)	1312877..1313755	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LJU33_RS06345 (1313772)	1313772..1314806	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
LJU33_RS06350 (1315019)	1315019..1315732	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
LJU33_RS06355 (1315887)	1315887..1316114	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
LJU33_RS06360 (1316114)	1316114..1316512	+	399	WP_000911327.1	type II toxin-antitoxin system VapC family toxin	Toxin
LJU33_RS06365 (1316659)	1316659..1317522	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
LJU33_RS06370 (1317537)	1317537..1319552	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
LJU33_RS06375 (1319626)	1319626..1320324	+	699	WP_000679823.1	esterase	-
LJU33_RS06380 (1320434)	1320434..1320634	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T221005 WP_000911327.1 NZ_CP085637:1316114-1316512 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMEIIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T221005 NZ_CP117327:c3987107-3986820 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT221005 WP_000450524.1 NZ_CP085637:1315887-1316114 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT221005 NZ_CP117327:c3986833-3986531 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33