Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 853184..853838 Replicon chromosome
Accession NZ_CP085637
Organism Escherichia coli strain fECg99.1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag LJU33_RS04195 Protein ID WP_000244772.1
Coordinates 853431..853838 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LJU33_RS04190 Protein ID WP_000354046.1
Coordinates 853184..853450 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJU33_RS04165 (848472) 848472..849215 + 744 WP_000951964.1 SDR family oxidoreductase -
LJU33_RS04170 (849272) 849272..850705 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LJU33_RS04175 (850750) 850750..851061 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
LJU33_RS04180 (851225) 851225..851884 + 660 WP_000250274.1 hemolysin III family protein -
LJU33_RS04185 (851961) 851961..852941 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LJU33_RS04190 (853184) 853184..853450 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LJU33_RS04195 (853431) 853431..853838 + 408 WP_000244772.1 protein YgfX Toxin
LJU33_RS04200 (853878) 853878..854399 - 522 WP_001055874.1 flavodoxin FldB -
LJU33_RS04205 (854511) 854511..855407 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LJU33_RS04210 (855432) 855432..856142 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LJU33_RS04215 (856148) 856148..857881 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T221003 WP_000244772.1 NZ_CP085637:853431-853838 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T221003 NZ_CP117327:3381971-3382189 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT221003 WP_000354046.1 NZ_CP085637:853184-853450 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT221003 NZ_CP117327:3381569-3381943 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References