Detailed information of TA system TA220997

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 2222521..2223334
• Replicon: chromosome
• Accession: NZ_CP085636
• Organism: Shewanella sp. MTB7

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: HWQ47_RS09440
• Protein ID: WP_269970883.1
• Coordinates: 2222939..2223334 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: HWQ47_RS09435
• Protein ID: WP_269970882.1
• Coordinates: 2222521..2222946 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ47_RS09425 (HWQ47_09425)	2217932..2219035	+	1104	WP_269970880.1	response regulator	-
HWQ47_RS09430 (HWQ47_09430)	2219804..2221276	-	1473	WP_269970881.1	peptide MFS transporter	-
HWQ47_RS09435 (HWQ47_09435)	2222521..2222946	+	426	WP_269970882.1	nucleotidyltransferase domain-containing protein	Antitoxin
HWQ47_RS09440 (HWQ47_09440)	2222939..2223334	+	396	WP_269970883.1	DUF86 domain-containing protein	Toxin
HWQ47_RS09445 (HWQ47_09445)	2224038..2224574	+	537	WP_269970884.1	transcription/translation regulatory transformer protein RfaH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-130)

Antitoxin

• Polymerase beta, nucleotidyltransferase (14-97)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15105.33 Da
• Isoelectric Point: 7.8466

>T220997 WP_269970883.1 NZ_CP085636:2222939-2223334 [Shewanella sp. MTB7]
MNDIIINKIATINRCLKRIKDVYGNGIAFRKDHTQQDSVVLNLQRTCEACIDIANHLNRKHQYGVPQSSRDSFTLLAQNN
VLPDELAENLKKMVGLRNIAVHDYQELNLDIVVHVINHRLSDFERFIEAIK

Nucleotide

• Length: 396 bp

>T220997 NZ_CP117327:364793-365068 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGGGCAAAGGAAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGGGCCTGACTGGATTCTTATTGATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15891.27 Da
• Isoelectric Point: 4.4336

>AT220997 WP_269970882.1 NZ_CP085636:2222521-2222946 [Shewanella sp. MTB7]
MTMFNLDKSRLINLVLDKIPTVKLIYLFGSYASGEQHSKSDLDIAILPVKSLDNLSRWQLAQTLACELDIDVDLIDLTSA
STVLCQQVITQGRLLWGDSYDDDIFAVKTISMYQNLQVERAMILADLMNKDPRDGRGTVHE

Nucleotide

• Length: 426 bp

>AT220997 NZ_CP117327:364530-364790 [Salmonella enterica subsp. enterica serovar Saintpaul]
ATGGCTGCAAATGTGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGAATCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGGATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGACATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA