Detailed information of TA system TA220990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2944077..2944711
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LJQ72_RS13240
• Protein ID: WP_227526939.1
• Coordinates: 2944077..2944481 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LJQ72_RS13245
• Protein ID: WP_104209861.1
• Coordinates: 2944481..2944711 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS13220 (LJQ72_13220)	2940720..2940938	+	219	WP_010279476.1	KTSC domain-containing protein	-
LJQ72_RS13225 (LJQ72_13225)	2941163..2941519	+	357	WP_227526936.1	hypothetical protein	-
LJQ72_RS13230 (LJQ72_13230)	2941574..2942473	-	900	WP_227526937.1	LysR family transcriptional regulator	-
LJQ72_RS13235 (LJQ72_13235)	2942585..2943598	+	1014	WP_227526938.1	CAR family subclass B3 metallo-beta-lactamase	-
LJQ72_RS13240 (LJQ72_13240)	2944077..2944481	-	405	WP_227526939.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LJQ72_RS13245 (LJQ72_13245)	2944481..2944711	-	231	WP_104209861.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LJQ72_RS13250 (LJQ72_13250)	2944806..2945639	-	834	WP_227526940.1	DUF4942 domain-containing protein	-
LJQ72_RS13255 (LJQ72_13255)	2945757..2946077	-	321	WP_227526941.1	TA system toxin CbtA family protein	-
LJQ72_RS13260 (LJQ72_13260)	2946147..2946482	-	336	WP_227526942.1	type IV toxin-antitoxin system YeeU family antitoxin	-
LJQ72_RS13265 (LJQ72_13265)	2946522..2946995	-	474	WP_227526943.1	DNA repair protein RadC	-
LJQ72_RS13270 (LJQ72_13270)	2947118..2947468	-	351	WP_227526944.1	hypothetical protein	-
LJQ72_RS13275 (LJQ72_13275)	2947526..2948359	-	834	WP_227526945.1	hypothetical protein	-
LJQ72_RS13280 (LJQ72_13280)	2948506..2948664	-	159	WP_155242703.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15058.30 Da
• Isoelectric Point: 8.8008

>T220990 WP_227526939.1 NZ_CP085633:c2944481-2944077 [Pectobacterium brasiliense]
MLKYLLDTNICIYTIKNKPKEVRDAFYRHYGQFAISSITLMELIYGAEKSVNPEKNLAVIEGFSARLEVQNYGFDAAVHT
GQIRAELARQGTPIGPYDAMLAGHARSAGLILVTNNVREFERVPGLRVENWVGR

Nucleotide

• Length: 405 bp

>T220990 NZ_CP117326:3612627-3612902 [Salmonella enterica subsp. enterica serovar Newport]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACCGTGAAGATCTCCAAGGGATGTT
TGATCCTGATGGCTGAGACGGACGAAGTGAGGGAGCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATGATGTGGTGAACGGGGATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8666.74 Da
• Isoelectric Point: 4.4230

>AT220990 WP_104209861.1 NZ_CP085633:c2944711-2944481 [Pectobacterium brasiliense]
MEKTTVFKSNRSQAVRLPKAVALPDDVKQVDIVAIGRTRIITPAGESWDSWFEGESVTPDFMITREQPDEQVREDF

Nucleotide

• Length: 231 bp

>AT220990 NZ_CP117326:c3612892-3612470 [Salmonella enterica subsp. enterica serovar Newport]
TTCACCACATCATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGCTCCCTCAC
TTCGTCCGTCTCAGCCATCAGGATCAAACATCCCTTGGAGATCTTCACGGTCACGCTGCGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGTACCGGGCGGTGATGAACA
ACCGGCTACCGGGGCCGTATTTT