Detailed information of TA system TA220987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1004416..1005452
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LJQ72_RS04510
• Protein ID: WP_235204923.1
• Coordinates: 1004949..1005452 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A6P1RQC6
• Locus tag: LJQ72_RS04505
• Protein ID: WP_039497370.1
• Coordinates: 1004416..1004877 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS04485 (LJQ72_04485)	999478..1000011	+	534	WP_010282870.1	RNA pyrophosphohydrolase	-
LJQ72_RS04490 (LJQ72_04490)	1000024..1002270	+	2247	WP_227527786.1	phosphoenolpyruvate--protein phosphotransferase	-
LJQ72_RS04495 (LJQ72_04495)	1002497..1003363	+	867	WP_039313946.1	prolipoprotein diacylglyceryl transferase	-
LJQ72_RS04500 (LJQ72_04500)	1003400..1004194	+	795	WP_039312476.1	thymidylate synthase	-
LJQ72_RS04505 (LJQ72_04505)	1004416..1004877	+	462	WP_039497370.1	helix-turn-helix domain-containing protein	Antitoxin
LJQ72_RS04510 (LJQ72_04510)	1004949..1005452	+	504	WP_235204923.1	PIN domain-containing protein	Toxin
LJQ72_RS04515 (LJQ72_04515)	1005632..1006135	+	504	WP_227527787.1	prepilin peptidase-dependent protein	-
LJQ72_RS04520 (LJQ72_04520)	1006129..1006755	+	627	WP_227527788.1	prepilin peptidase-dependent protein	-
LJQ72_RS04525 (LJQ72_04525)	1006752..1007207	+	456	WP_227527789.1	YgdB family protein	-
LJQ72_RS04530 (LJQ72_04530)	1007194..1007589	+	396	WP_227528263.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-91)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18752.65 Da
• Isoelectric Point: 4.6914

>T220987 WP_235204923.1 NZ_CP085633:1004949-1005452 [Pectobacterium brasiliense]
MHLGLTGLYQPKWTALIQEEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGFEQLIPALVLPDADDRHVLAAAIRA
NAEVIVTANLKDFPATALSEFAVEAMHPDEFIADLFDLNSALVLKAVRTSRTAFRKPPFDVDEYLDCLLRQGLPMTVKEL
DAYRALI

Nucleotide

• Length: 504 bp

>T220987 NZ_CP117326:c1002395-1001868 [Salmonella enterica subsp. enterica serovar Newport]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17243.76 Da
• Isoelectric Point: 6.2253

>AT220987 WP_039497370.1 NZ_CP085633:1004416-1004877 [Pectobacterium brasiliense]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISIQDAHDIAHRIELPTSALTLLMSILGELAAGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRENESMQAMQELTDRSQELGFY

Nucleotide

• Length: 462 bp

>AT220987 NZ_CP117326:c1002682-1002392 [Salmonella enterica subsp. enterica serovar Newport]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1RQC6