Detailed information of TA system TA220985

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 734925..735585
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: LJQ72_RS03310
• Protein ID: WP_010301815.1
• Coordinates: 735211..735585 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: LJQ72_RS03305
• Protein ID: WP_005971623.1
• Coordinates: 734925..735191 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS03285 (LJQ72_03285)	730889..731893	-	1005	WP_205534120.1	LacI family DNA-binding transcriptional regulator	-
LJQ72_RS03290 (LJQ72_03290)	731948..732556	-	609	WP_014914174.1	HD domain-containing protein	-
LJQ72_RS03295 (LJQ72_03295)	732986..733633	+	648	WP_010279833.1	hemolysin III family protein	-
LJQ72_RS03300 (LJQ72_03300)	733688..734689	-	1002	WP_205534119.1	tRNA-modifying protein YgfZ	-
LJQ72_RS03305 (LJQ72_03305)	734925..735191	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
LJQ72_RS03310 (LJQ72_03310)	735211..735585	+	375	WP_010301815.1	protein YgfX	Toxin
LJQ72_RS03315 (LJQ72_03315)	735654..736589	+	936	WP_010301818.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
LJQ72_RS03320 (LJQ72_03320)	736655..736978	-	324	WP_227527698.1	hypothetical protein	-
LJQ72_RS03325 (LJQ72_03325)	737007..737525	-	519	WP_227527699.1	flavodoxin FldB	-
LJQ72_RS03330 (LJQ72_03330)	737869..738255	-	387	WP_039282122.1	thioesterase family protein	-
LJQ72_RS03335 (LJQ72_03335)	738460..739788	-	1329	WP_039282120.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-119)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14654.33 Da
• Isoelectric Point: 10.5560

>T220985 WP_010301815.1 NZ_CP085633:735211-735585 [Pectobacterium brasiliense]
MQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQEWQIVKRPWLLKN
GVLLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 375 bp

>T220985 NZ_CP117326:836562-836975 [Salmonella enterica subsp. enterica serovar Newport]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT220985 WP_005971623.1 NZ_CP085633:734925-735191 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT220985 NZ_CP117326:836315-836581 [Salmonella enterica subsp. enterica serovar Newport]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAATTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6