Detailed information of TA system TA220981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 390348..391187
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LJQ72_RS01815
• Protein ID: WP_039510070.1
• Coordinates: 390825..391187 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LJQ72_RS01810
• Protein ID: WP_227527600.1
• Coordinates: 390348..390821 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS01790 (LJQ72_01790)	385448..386371	-	924	WP_227527597.1	aminoimidazole riboside kinase	-
LJQ72_RS01795 (LJQ72_01795)	386695..386883	+	189	WP_180743175.1	hypothetical protein	-
LJQ72_RS01800 (LJQ72_01800)	387053..387604	+	552	WP_227527598.1	polymer-forming cytoskeletal protein	-
LJQ72_RS01805 (LJQ72_01805)	387641..390358	+	2718	WP_227527599.1	CRISPR-associated helicase Cas3'	-
LJQ72_RS01810 (LJQ72_01810)	390348..390821	-	474	WP_227527600.1	hypothetical protein	Antitoxin
LJQ72_RS01815 (LJQ72_01815)	390825..391187	-	363	WP_039510070.1	type II toxin-antitoxin system HigB family toxin	Toxin
LJQ72_RS01820 (LJQ72_01820)	391798..393366	+	1569	WP_227527601.1	type I-E CRISPR-associated protein Cse1/CasA	-
LJQ72_RS01825 (LJQ72_01825)	393350..393997	+	648	WP_039524282.1	type I-E CRISPR-associated protein Cse2/CasB	-
LJQ72_RS01830 (LJQ72_01830)	394001..395065	+	1065	WP_227527602.1	type I-E CRISPR-associated protein Cas7/Cse4/CasC	-
LJQ72_RS01835 (LJQ72_01835)	395075..395809	+	735	WP_227527603.1	type I-E CRISPR-associated protein Cas5/CasD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (100-155)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14242.46 Da
• Isoelectric Point: 9.7477

>T220981 WP_039510070.1 NZ_CP085633:c391187-390825 [Pectobacterium brasiliense]
MRVISAKAITDAMKKHAKWRVGLKLWLDVFDKPSLRFESYEQLCHVWRNTSCWNVDRIPFTLLENESRKGPLDIYVFDIH
GNNCRIIAWLNPNTGSLYVKDVCSHAEYDKWWKAQTKTKR

Nucleotide

• Length: 363 bp

>T220981 NZ_CP117322:c4276791-4276300 [Salmonella enterica subsp. enterica serovar Derby]
ATGATCTCCCCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGCCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATCCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17850.23 Da
• Isoelectric Point: 4.2779

>AT220981 WP_227527600.1 NZ_CP085633:c390821-390348 [Pectobacterium brasiliense]
MRNTSLGSNSPVGISGLIENLPLLLQEASEQLSSLSVLLTACMEPIEDDKDLQERMECINQLYLYSSDVNDIPATFAELI
ADRVYEYEKKNRDVPHVSQAEALAFFIEERRLKQSDLKQIATQSVISDIINGKRTMTLQQVKDFSRYFNVPVETFID

Nucleotide

• Length: 474 bp

>AT220981 NZ_CP117322:c4277081-4276788 [Salmonella enterica subsp. enterica serovar Derby]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGTACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA