Detailed information of TA system TA220980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 339385..339916
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: LJQ72_RS01590
• Protein ID: WP_227527582.1
• Coordinates: 339629..339916 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A1V2R9J2
• Locus tag: LJQ72_RS01585
• Protein ID: WP_039282788.1
• Coordinates: 339385..339639 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS01560 (LJQ72_01560)	334695..335285	-	591	WP_005975525.1	DnaA initiator-associating protein DiaA	-
LJQ72_RS01565 (LJQ72_01565)	335349..335744	-	396	WP_072009247.1	YraN family protein	-
LJQ72_RS01570 (LJQ72_01570)	335702..337720	-	2019	WP_227527581.1	penicillin-binding protein activator	-
LJQ72_RS01575 (LJQ72_01575)	337782..338669	+	888	WP_039282792.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
LJQ72_RS01585 (LJQ72_01585)	339385..339639	+	255	WP_039282788.1	plasmid stabilization protein	Antitoxin
LJQ72_RS01590 (LJQ72_01590)	339629..339916	+	288	WP_227527582.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LJQ72_RS01595 (LJQ72_01595)	340093..340878	+	786	WP_227527583.1	4,5-DOPA dioxygenase extradiol	-
LJQ72_RS01600 (LJQ72_01600)	340946..342106	-	1161	WP_227527584.1	glutathionylspermidine synthase family protein	-
LJQ72_RS01605 (LJQ72_01605)	342118..342792	-	675	WP_010280216.1	DUF1190 family protein	-
LJQ72_RS01610 (LJQ72_01610)	343063..344457	-	1395	WP_039499674.1	outer membrane channel protein TolC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11025.95 Da
• Isoelectric Point: 10.8186

>T220980 WP_227527582.1 NZ_CP085633:339629-339916 [Pectobacterium brasiliense]
MTYKLKFVPSAMKEWKKLGHPVREQFKKKLVERLENPRVPSAQLHGRKDQYKIKLRSAGYRLVYLVQDETITVMVMGVGK
REGSQVYSDTKGRSA

Nucleotide

• Length: 288 bp

>T220980 NZ_CP085633:339629-339916 [Pectobacterium brasiliense]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGCTAGGGCATCCTGTCCGGGAACAGTTCAA
GAAAAAGCTGGTTGAGCGTTTGGAAAATCCTCGTGTTCCTTCAGCCCAGCTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAGGTCCGCTGGCTACAGGCTGGTGTATTTGGTCCAGGATGAGACCATTACTGTGATGGTTATGGGAGTTGGAAAG
CGCGAAGGTAGCCAGGTTTATTCTGATACGAAAGGTCGCTCTGCTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9208.38 Da
• Isoelectric Point: 3.7947

>AT220980 WP_039282788.1 NZ_CP085633:339385-339639 [Pectobacterium brasiliense]
MAFNILTNTAASITELKRDPMGTVRAAEGETIAILNRNEPAFYCVPPAVFAYLMELAEDAELGRTVDDRMSELADAEEIS
LDDL

Nucleotide

• Length: 255 bp

>AT220980 NZ_CP085633:339385-339639 [Pectobacterium brasiliense]
ATGGCTTTCAACATCCTAACTAACACAGCCGCCAGTATTACTGAGTTGAAACGTGACCCGATGGGGACGGTTCGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAGCCGGCGTTCTACTGTGTGCCTCCTGCTGTATTCGCCTATTTAA
TGGAACTGGCTGAAGATGCAGAACTGGGGCGTACCGTTGATGATCGGATGTCAGAGCTAGCTGACGCGGAAGAGATTTCG
CTCGATGACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2R9J2