Detailed information of TA system TA220979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 150547..151181
• Replicon: chromosome
• Accession: NZ_CP085633
• Organism: Pectobacterium brasiliense strain TS20HJ1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LJQ72_RS00685
• Protein ID: WP_080162911.1
• Coordinates: 150547..150822 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: LJQ72_RS00690
• Protein ID: WP_014917022.1
• Coordinates: 150819..151181 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJQ72_RS00670 (LJQ72_00670)	147567..148937	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
LJQ72_RS00675 (LJQ72_00675)	148934..149632	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
LJQ72_RS00680 (LJQ72_00680)	149786..150310	+	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
LJQ72_RS00685 (LJQ72_00685)	150547..150822	+	276	WP_080162911.1	type II toxin-antitoxin system HicA family toxin	Toxin
LJQ72_RS00690 (LJQ72_00690)	150819..151181	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LJQ72_RS00695 (LJQ72_00695)	151555..152742	+	1188	WP_039502362.1	sugar transporter	-
LJQ72_RS00700 (LJQ72_00700)	152825..153727	+	903	WP_039502361.1	CDF family cation-efflux transporter FieF	-
LJQ72_RS00705 (LJQ72_00705)	153935..154897	+	963	WP_010306854.1	6-phosphofructokinase	-
LJQ72_RS00710 (LJQ72_00710)	155178..156062	+	885	WP_039465389.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10024.61 Da
• Isoelectric Point: 11.1875

>T220979 WP_080162911.1 NZ_CP085633:150547-150822 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T220979 NZ_CP117322:c4084263-4083955 [Salmonella enterica subsp. enterica serovar Derby]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGGTTAGTAAATAACCCAGTTAAGCGTCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT220979 WP_014917022.1 NZ_CP085633:150819-151181 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT220979 NZ_CP117322:c4084505-4084266 [Salmonella enterica subsp. enterica serovar Derby]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACGGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7