Detailed information of TA system TA220975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3064949..3065475
• Replicon: chromosome
• Accession: NZ_CP085627
• Organism: Erwinia rhapontici strain BY21311

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LJN55_RS14335
• Protein ID: WP_133841673.1
• Coordinates: 3065170..3065475 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: LJN55_RS14330
• Protein ID: WP_227534684.1
• Coordinates: 3064949..3065167 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN55_RS14310 (LJN55_14310)	3060321..3062135	-	1815	WP_227534682.1	hypothetical protein	-
LJN55_RS14315 (LJN55_14315)	3062192..3062707	-	516	WP_085396429.1	hypothetical protein	-
LJN55_RS14320 (LJN55_14320)	3062704..3063888	-	1185	WP_227534683.1	restriction endonuclease	-
LJN55_RS14325 (LJN55_14325)	3064560..3064811	-	252	WP_159336428.1	hypothetical protein	-
LJN55_RS14330 (LJN55_14330)	3064949..3065167	+	219	WP_227534684.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LJN55_RS14335 (LJN55_14335)	3065170..3065475	+	306	WP_133841673.1	type II toxin-antitoxin system toxin CcdB	Toxin
LJN55_RS14340 (LJN55_14340)	3066120..3067508	-	1389	WP_227534685.1	ATP-binding protein	-
LJN55_RS14345 (LJN55_14345)	3067508..3068185	-	678	WP_227534686.1	response regulator	-
LJN55_RS14350 (LJN55_14350)	3068390..3069226	+	837	WP_227534687.1	MipA/OmpV family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11670.63 Da
• Isoelectric Point: 6.8688

>T220975 WP_133841673.1 NZ_CP085627:3065170-3065475 [Erwinia rhapontici]
MQFIVYQYKRTSHYKIFVDVQSDIVDTPGRRMVIPLIESHHLSEKVNKTLFPLILIEGKNHRLMTTELSSVPVEVIGDAV
VDITRCQDDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T220975 NZ_CP117322:c2325185-2324901 [Salmonella enterica subsp. enterica serovar Derby]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8191.16 Da
• Isoelectric Point: 5.8395

>AT220975 WP_227534684.1 NZ_CP085627:3064949-3065167 [Erwinia rhapontici]
MKHRVSVTVDKENYLVLNAAGVNISGLVNDVIGREARRIKAEEWKEQNREGMEEVASFIAKNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT220975 NZ_CP117322:c2325423-2325175 [Salmonella enterica subsp. enterica serovar Derby]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA