Detailed information of TA system TA220972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1119418..1120046
• Replicon: chromosome
• Accession: NZ_CP085627
• Organism: Erwinia rhapontici strain BY21311

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: LJN55_RS05240
• Protein ID: WP_133845760.1
• Coordinates: 1119418..1119636 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LJN55_RS05245
• Protein ID: WP_133845759.1
• Coordinates: 1119666..1120046 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN55_RS05210 (LJN55_05210)	1115385..1115723	+	339	WP_227535796.1	P-II family nitrogen regulator	-
LJN55_RS05215 (LJN55_05215)	1115743..1117044	+	1302	WP_171148780.1	ammonium transporter AmtB	-
LJN55_RS05220 (LJN55_05220)	1117139..1117999	-	861	WP_133845762.1	acyl-CoA thioesterase II	-
LJN55_RS05225 (LJN55_05225)	1118225..1118782	+	558	WP_133845761.1	YbaY family lipoprotein	-
LJN55_RS05230 (LJN55_05230)	1118812..1119126	-	315	WP_133845785.1	MGMT family protein	-
LJN55_RS05240 (LJN55_05240)	1119418..1119636	-	219	WP_133845760.1	HHA domain-containing protein	Toxin
LJN55_RS05245 (LJN55_05245)	1119666..1120046	-	381	WP_133845759.1	Hha toxicity modulator TomB	Antitoxin
LJN55_RS05250 (LJN55_05250)	1120194..1120547	-	354	WP_159337511.1	hypothetical protein	-
LJN55_RS23905	1120834..1120959	+	126	WP_259151068.1	hypothetical protein	-
LJN55_RS05255 (LJN55_05255)	1120959..1121648	+	690	WP_133845757.1	ATP-binding cassette domain-containing protein	-
LJN55_RS05260 (LJN55_05260)	1121645..1122508	+	864	WP_133845756.1	metal ABC transporter permease	-
LJN55_RS05265 (LJN55_05265)	1122511..1123389	+	879	WP_133845755.1	metal ABC transporter substrate-binding protein	-
LJN55_RS05270 (LJN55_05270)	1123504..1123647	-	144	WP_012442195.1	type B 50S ribosomal protein L36	-
LJN55_RS05275 (LJN55_05275)	1123657..1123917	-	261	WP_133845754.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8655.11 Da
• Isoelectric Point: 8.9007

>T220972 WP_133845760.1 NZ_CP085627:c1119636-1119418 [Erwinia rhapontici]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAIFYSAADHRLAELTMNKLYDKIPVAVWKFVR

Nucleotide

• Length: 219 bp

>T220972 NZ_CP117322:352390-352665 [Salmonella enterica subsp. enterica serovar Derby]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATTACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGGGCCTGACTGGATTCTTATTGATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14562.10 Da
• Isoelectric Point: 4.5911

>AT220972 WP_133845759.1 NZ_CP085627:c1120046-1119666 [Erwinia rhapontici]
MDEYSPRRHDIAQLKFLSESLYDESLATLGDSHHGWVNDPTSAANLQLNDLIEHIAAFTMNYKIKHIEDSDLITQVDEYL
DDTFMLFSNYGINAQDLQRWQKSAKRLFNIFAEECATSRTQASHSF

Nucleotide

• Length: 381 bp

>AT220972 NZ_CP117322:352127-352387 [Salmonella enterica subsp. enterica serovar Derby]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGAATCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGGATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGACATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA