Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 88500..90123 Replicon plasmid pEC71-IncHI2
Accession NZ_CP085623
Organism Escherichia coli strain EC71

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag LJN53_RS23460 Protein ID WP_000088046.1
Coordinates 88798..90123 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag LJN53_RS23455 Protein ID WP_024136327.1
Coordinates 88500..88793 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN53_RS23430 (LJN53_23430) 84372..85613 + 1242 WP_001053338.1 VWA domain-containing protein -
LJN53_RS25685 85704..85835 + 132 WP_000374059.1 hypothetical protein -
LJN53_RS23435 (LJN53_23435) 86176..86457 - 282 WP_000077926.1 hypothetical protein -
LJN53_RS23440 (LJN53_23440) 86507..86698 - 192 WP_000398480.1 hypothetical protein -
LJN53_RS23445 (LJN53_23445) 86790..87131 - 342 WP_001151575.1 hypothetical protein -
LJN53_RS23450 (LJN53_23450) 87504..87896 + 393 WP_000341066.1 hypothetical protein -
LJN53_RS23455 (LJN53_23455) 88500..88793 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
LJN53_RS23460 (LJN53_23460) 88798..90123 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
LJN53_RS23465 (LJN53_23465) 90184..90390 + 207 WP_000134171.1 hypothetical protein -
LJN53_RS23470 (LJN53_23470) 90492..90902 + 411 WP_000985911.1 hypothetical protein -
LJN53_RS23475 (LJN53_23475) 90915..91730 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
LJN53_RS23480 (LJN53_23480) 91984..92409 + 426 WP_001043843.1 hypothetical protein -
LJN53_RS23485 (LJN53_23485) 92958..93266 + 309 WP_001224687.1 hypothetical protein -
LJN53_RS23490 (LJN53_23490) 93282..94139 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
LJN53_RS23495 (LJN53_23495) 94201..94404 + 204 WP_001194555.1 hypothetical protein -
LJN53_RS23500 (LJN53_23500) 94746..95102 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / floR / sul3 / tet(A) / aph(6)-Id / lnu(F) / ant(3'')-Ia / aac(3)-IId / blaLAP-2 / blaTEM-1B / dfrA14 / ARR-3 / mph(A) / qnrS1 / blaCTX-M-55 - 1..269592 269592


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T220960 WP_000088046.1 NZ_CP085623:88798-90123 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T220960 NZ_CP117317:c980823-980296 [Salmonella enterica subsp. enterica serovar Derby]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATACACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT220960 WP_024136327.1 NZ_CP085623:88500-88793 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT220960 NZ_CP117317:c981110-980820 [Salmonella enterica subsp. enterica serovar Derby]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References