Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4298294..4298791 Replicon chromosome
Accession NZ_CP085622
Organism Escherichia coli strain EC71

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LJN53_RS20735 Protein ID WP_001173343.1
Coordinates 4298294..4298467 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LJN53_RS20740 Protein ID WP_000398619.1
Coordinates 4298495..4298791 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN53_RS20725 (4294964) 4294964..4296421 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
LJN53_RS20730 (4296658) 4296658..4298175 + 1518 WP_001295074.1 lysine--tRNA ligase -
LJN53_RS20735 (4298294) 4298294..4298467 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LJN53_RS20740 (4298495) 4298495..4298791 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LJN53_RS20745 (4299018) 4299018..4299290 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LJN53_RS20750 (4299302) 4299302..4299532 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
LJN53_RS20755 (4299713) 4299713..4301344 + 1632 WP_001216461.1 sensor histidine kinase -
LJN53_RS20760 (4301341) 4301341..4302060 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T220958 WP_001173343.1 NZ_CP085622:c4298467-4298294 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T220958 NZ_CP117317:351481-351756 [Salmonella enterica subsp. enterica serovar Derby]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATTACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGGGCCTGACTGGATTCTTATTGATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT220958 WP_000398619.1 NZ_CP085622:c4298791-4298495 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT220958 NZ_CP117317:351218-351478 [Salmonella enterica subsp. enterica serovar Derby]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGAATCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGGATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGACATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References