220954

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3462380..3463217 Replicon chromosome
Accession NZ_CP085622
Organism Escherichia coli strain EC71

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag LJN53_RS16845 Protein ID WP_000227784.1
Coordinates 3462675..3463217 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag LJN53_RS16840 Protein ID WP_001297137.1
Coordinates 3462380..3462691 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN53_RS16815 (3457400) 3457400..3458347 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
LJN53_RS16820 (3458369) 3458369..3460360 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
LJN53_RS16825 (3460350) 3460350..3460964 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LJN53_RS16830 (3460964) 3460964..3461293 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LJN53_RS16835 (3461305) 3461305..3462195 + 891 WP_000971336.1 heme o synthase -
LJN53_RS16840 (3462380) 3462380..3462691 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
LJN53_RS16845 (3462675) 3462675..3463217 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
LJN53_RS16850 (3463273) 3463273..3464208 - 936 WP_001297127.1 tetratricopeptide repeat protein -
LJN53_RS16855 (3464616) 3464616..3465980 + 1365 WP_001000978.1 MFS transporter -
LJN53_RS16860 (3466108) 3466108..3466599 - 492 WP_001138904.1 nucleotide binding protein YajQ -
LJN53_RS16865 (3466767) 3466767..3467678 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T220954 WP_000227784.1 NZ_CP085622:3462675-3463217 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T220954 NZ_CP117312:2853352-2853455 [Salmonella enterica subsp. enterica serovar Derby]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT220954 WP_001297137.1 NZ_CP085622:3462380-3462691 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT220954 NZ_CP117312:c2853457-2853314 [Salmonella enterica subsp. enterica serovar Derby]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References