Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3428303..3428921 Replicon chromosome
Accession NZ_CP085622
Organism Escherichia coli strain EC71

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LJN53_RS16675 Protein ID WP_001291435.1
Coordinates 3428703..3428921 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LJN53_RS16670 Protein ID WP_000344800.1
Coordinates 3428303..3428677 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN53_RS16660 (3423392) 3423392..3424585 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LJN53_RS16665 (3424608) 3424608..3427757 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LJN53_RS16670 (3428303) 3428303..3428677 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LJN53_RS16675 (3428703) 3428703..3428921 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LJN53_RS16680 (3429093) 3429093..3429644 + 552 WP_000102568.1 maltose O-acetyltransferase -
LJN53_RS16685 (3429760) 3429760..3430230 + 471 WP_000136192.1 YlaC family protein -
LJN53_RS16690 (3430394) 3430394..3431944 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LJN53_RS16695 (3431986) 3431986..3432339 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LJN53_RS16705 (3432718) 3432718..3433029 + 312 WP_000409911.1 MGMT family protein -
LJN53_RS16710 (3433060) 3433060..3433632 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T220953 WP_001291435.1 NZ_CP085622:3428703-3428921 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T220953 NZ_CP117312:c2141499-2141281 [Salmonella enterica subsp. enterica serovar Derby]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT220953 WP_000344800.1 NZ_CP085622:3428303-3428677 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT220953 NZ_CP117312:c2141901-2141527 [Salmonella enterica subsp. enterica serovar Derby]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGGTTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References