Detailed information of TA system TA220952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2447810..2448448
• Replicon: chromosome
• Accession: NZ_CP085622
• Organism: Escherichia coli strain EC71

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LJN53_RS11900
• Protein ID: WP_000813794.1
• Coordinates: 2448272..2448448 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LJN53_RS11895
• Protein ID: WP_001270286.1
• Coordinates: 2447810..2448226 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJN53_RS11875 (2442962)	2442962..2443903	-	942	WP_001251319.1	ABC transporter permease	-
LJN53_RS11880 (2443904)	2443904..2444917	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LJN53_RS11885 (2444935)	2444935..2446080	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
LJN53_RS11890 (2446325)	2446325..2447731	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
LJN53_RS11895 (2447810)	2447810..2448226	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LJN53_RS11900 (2448272)	2448272..2448448	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LJN53_RS11905 (2448670)	2448670..2448900	+	231	WP_000494244.1	YncJ family protein	-
LJN53_RS11910 (2448992)	2448992..2450953	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LJN53_RS11915 (2451026)	2451026..2451562	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
LJN53_RS11920 (2451654)	2451654..2452829	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T220952 WP_000813794.1 NZ_CP085622:c2448448-2448272 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T220952 NZ_CP117312:c1913003-1912728 [Salmonella enterica subsp. enterica serovar Derby]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGCGACTGGCTGGCAGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGAGCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATGATGTGGTGAACGGGAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT220952 WP_001270286.1 NZ_CP085622:c2448226-2447810 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT220952 NZ_CP117312:1912738-1913150 [Salmonella enterica subsp. enterica serovar Derby]
TTCACCACATCATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGCTCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATGTGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCCCCCTCTCCCCGGCCAGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Structures

Toxin

• PDB: 6HPB