Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 270364..270950 Replicon chromosome
Accession NZ_CP085622
Organism Escherichia coli strain EC71

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag LJN53_RS01215 Protein ID WP_042014162.1
Coordinates 270364..270594 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag LJN53_RS01220 Protein ID WP_000593555.1
Coordinates 270591..270950 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN53_RS01205 (266401) 266401..269136 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
LJN53_RS01210 (269136) 269136..270260 + 1125 WP_001314210.1 ABC transporter permease -
LJN53_RS01215 (270364) 270364..270594 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
LJN53_RS01220 (270591) 270591..270950 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LJN53_RS01225 (271070) 271070..271471 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
LJN53_RS01230 (271477) 271477..272283 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
LJN53_RS01235 (272280) 272280..273044 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
LJN53_RS01240 (273044) 273044..273877 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
LJN53_RS01245 (273874) 273874..274818 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T220935 WP_042014162.1 NZ_CP085622:270364-270594 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T220935 NZ_CP117307:816117-816530 [Salmonella enterica subsp. enterica serovar Derby]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGCCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT220935 WP_000593555.1 NZ_CP085622:270591-270950 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT220935 NZ_CP117307:815870-816136 [Salmonella enterica subsp. enterica serovar Derby]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References