Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4070696..4071272 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LJN50_RS20015 Protein ID WP_001297643.1
Coordinates 4070985..4071272 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LJN50_RS20010 Protein ID WP_000063156.1
Coordinates 4070696..4070998 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS19995 (4067292) 4067292..4069442 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LJN50_RS20000 (4069492) 4069492..4069695 + 204 WP_000467859.1 YbdD/YjiX family protein -
LJN50_RS20005 (4069706) 4069706..4070662 + 957 WP_001297640.1 GTPase -
LJN50_RS20010 (4070696) 4070696..4070998 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LJN50_RS20015 (4070985) 4070985..4071272 - 288 WP_001297643.1 BrnT family toxin Toxin
LJN50_RS20020 (4071471) 4071471..4072168 - 698 Protein_3912 IS1 family transposase -
LJN50_RS20025 (4072225) 4072225..4072320 + 96 Protein_3913 dienelactone hydrolase family protein -
LJN50_RS20030 (4072466) 4072466..4073698 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
LJN50_RS20035 (4073739) 4073739..4075019 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4071471..4071680 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T220928 WP_001297643.1 NZ_CP085619:c4071272-4070985 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T220928 NZ_CP117305:3622028-3622303 [Salmonella enterica subsp. enterica serovar Derby]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGCGACTGGCTGGCAGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGAGCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATGATGTGGTGAACGGGAATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT220928 WP_000063156.1 NZ_CP085619:c4070998-4070696 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT220928 NZ_CP117305:c3622293-3621881 [Salmonella enterica subsp. enterica serovar Derby]
TTCACCACATCATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGCTCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATGTGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCCCCCTCTCCCCGGCCAGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References