Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3938973..3939523 Replicon chromosome
Accession NZ_CP085619
Organism Escherichia coli strain EC32

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag LJN50_RS19330 Protein ID WP_001160966.1
Coordinates 3938973..3939287 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LJN50_RS19335 Protein ID WP_000125566.1
Coordinates 3939290..3939523 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJN50_RS19305 (3934648) 3934648..3935934 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
LJN50_RS19310 (3935934) 3935934..3936923 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LJN50_RS19315 (3936920) 3936920..3937741 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LJN50_RS19320 (3937744) 3937744..3938121 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LJN50_RS19325 (3938128) 3938128..3938976 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LJN50_RS19330 (3938973) 3938973..3939287 - 315 WP_001160966.1 CcdB family protein Toxin
LJN50_RS19335 (3939290) 3939290..3939523 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LJN50_RS19340 (3939609) 3939609..3940088 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
LJN50_RS19345 (3940280) 3940280..3942142 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
LJN50_RS19350 (3942135) 3942135..3942665 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LJN50_RS19355 (3942773) 3942773..3944104 - 1332 WP_001183207.1 MFS transporter -
LJN50_RS19360 (3944163) 3944163..3944450 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T220927 WP_001160966.1 NZ_CP085619:c3939287-3938973 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T220927 NZ_CP117305:3393524-3393742 [Salmonella enterica subsp. enterica serovar Derby]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT220927 WP_000125566.1 NZ_CP085619:c3939523-3939290 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT220927 NZ_CP117305:3393122-3393496 [Salmonella enterica subsp. enterica serovar Derby]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGGTTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References